Measurements of infarct volumes were performed as previously described (Yang et al., 2017 (link)). Briefly, infarct volume was determined with 2, 3, 5-triphenyltetrazolium chloride (TTC) 72 h post-MCAO. Mouse brain tissue was sliced into thick sections (1-mm-thick coronal sections) and stained with a 2% solution of TTC for 20 min at 37°C, followed by fixation with 4% paraformaldehyde. TTC-stained sections were imaged and analyzed using Image Pro-Plus 5.1 analysis system (Media Cybernetics, New York, NY, United States). Lesion volumes were calculated using the following formula: [total infarct volume-(volume of intact ipsilateral hemisphere–volume of intact contralateral hemisphere)]/contralateral hemisphere volume × 100%.
Image pro plus 5.1 analysis system
Manufactured by Media Cybernetics
Sourced in United States
Image Pro-Plus 5.1 is a software-based image analysis system. It provides tools for capturing, processing, analyzing, and measuring digital images. The system is designed to work with a variety of microscopy and imaging hardware.
Automatically generated - may contain errors
3 protocols using image pro plus 5.1 analysis system
1
Quantifying Infarct Volume in Mouse MCAO
2
Quantifying Ischemic Brain Injury in Mice
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Measurements of infarct volumes were performed as previously described [13 (link)]. Briefly, infarct volume was determined with 2, 3, 5-triphenyltetrazolium chloride (TTC) 72 h post-MCAO (n = 6 in each group). Brain tissue was sliced into thick sections (1-mm-thick coronal sections) and stained with a 2% solution of TTC for 20 min at 37 °C, followed by fixation with 4% paraformaldehyde. TTC-stained sections were imaged and analyzed using Image Pro-Plus 5.1 analysis system (Media Cybernetics, NY, NY, USA). Lesion volumes were calculated using the following formula: [total infarct volume − (the volume of intact ipsilateral hemisphere–the volume of intact contralateral hemisphere)]/contralateral hemisphere volume × 100%.
3
Infarct Volume Quantification Protocol
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Following decapitation, brains were removed and sectioned into 2-mm thick slices. These slices were kept in the dark and stained with 2% TTC (Sigma-Aldrich; Merck KGaA) for 30 min at room temperature, and then fixed with 4% PFA for 20 min at room temperature (Thermo Fisher Scientific, Inc.). Subsequently images were captured under magnification, ×4 and ×40 (Leica Microsystems GmbH; Upright Microscope). The infarct volume was evaluated by Image Pro-Plus 5.1 analysis system (Media Cybernetics, Inc., Rockville, MD, USA).
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