On the day of the analysis, liver tissue was thawed on ice, weighed and cleansed with PBS (pH = 7.4). Afterward, homogenization took place. It was performed using a glass homogenizer (Omni International, Kennesaw, GA, USA) whilst all the samples were kept on ice. Subsequently, the resulting suspension was sonicated using an ultrasonic homogenizer (cell disrupter) (1800 J per sample, 20 s three times on ice) (UP 400S, Hielscher, Teltow, Germany). Next, the homogenated tissue underwent centrifugation for 30 min at a temperature of 4 °C and a speed of 12,000 g. The resultant supernatant was further analyzed.
Glass homogenizer
Manufactured by Omni International
Sourced in United States
The Glass Homogenizer is a laboratory equipment used for mechanical disruption and mixing of samples. It operates by forcing the sample through a narrow opening, creating shear forces that break down and homogenize the material.
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Lab products found in correlation
2 protocols using glass homogenizer
1
Liver Tissue Homogenization and Fractionation
2
Sciatic Nerve NGF Quantification
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Sciatic nerve tissues were homogenized in a cold 50 mM phosphate buffered saline (pH 7.4) by a glass homogenizer (Omni International, Kennesaw, GA, USA). The homogenate was centrifuged at 1000 rpm for 10 min at 4°C to isolate nuclei and unbroken cells. After discarding the pellets, a portion of supernatant was centrifuged again at 12000 rpm for 20 min to obtain post-mitochondrial supernatant. The sciatic nerve level of NGF was assessed and quantified using the enzyme-linked immunoabsorbent assay (ELISA) technique (USCN LIFE, Wuhan EIAab Science Co., Ltd, Wuhan, China) according to the manufacturer’s instructions.
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