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Cyclin b1 h 433

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Cyclin B1 (H-433) is a reagent produced by Santa Cruz Biotechnology. It is a rabbit polyclonal antibody that recognizes Cyclin B1, a regulatory protein involved in cell cycle progression.

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Lab products found in correlation

Anti src py416, by Cell Signaling Technology (1 mentions) Anti rabbit igg antibody, by GE Healthcare (1 mentions) α tubulin mca78g, by Bio-Rad (1 mentions) Hybond nitrocellulose membrane, by Cytiva (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions) Gapdh g 9, by Santa Cruz Biotechnology (1 mentions) Ha ha 7, by Merck Group (1 mentions) Phospho gsk3α β, by Cell Signaling Technology (1 mentions) Hpv18 e6 g 7, by Santa Cruz Biotechnology (1 mentions) Phospho akt ser473 d9e, by Cell Signaling Technology (1 mentions) Frs2α, by Cell Signaling Technology (1 mentions) Species specific hrp conjugated secondary antibodies, by Merck Group (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Hybond, by Cytiva (1 mentions) Gm130, by BD (1 mentions) Phospho aurora a thr288 aurora b thr232 aurora c thr198, by Cell Signaling Technology (1 mentions) P aurora a b c, by Cell Signaling Technology (1 mentions) Src py416 phospho src family, by Cell Signaling Technology (1 mentions) Lamp1, by Santa Cruz Biotechnology (1 mentions) Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions)

Spelling variants of this product

Cyclin B1 (162 citations) H-433 (2 citations) Cyclin B1 (H-433 sc-752; (2 citations) Anti-cyclin B1 (H 433) (2 citations)

4 protocols using cyclin b1 h 433

1

Antibody Panel for Cell Cycle Analysis

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The following antibodies are used: Lyn (clone 9, Wako; H-6, sc-7274, Santa Cruz Biotechnology), actin (C4, Millipore; 13E5, Cell Signaling Technology), phosphotyrosine (pTyr) (4G10, Upstate Biotechnology Inc.), Src (#327, Calbiochem), anti-Src[pY416] (phospho-Src family, Cell Signaling Technology), cyclin B1 (H-433, Santa Cruz Biotechnology; V152, Cell Signaling Technology), phospho-histone H3 serine at position 10 (H3pS10) (clone 6G3, #9706 S, Cell Signaling Technology), and α-tubulin (MCA78G, Serotec). Horseradish peroxidase (HRP)-F(ab′)2 fragments of anti-mouse IgG antibody, anti-rabbit IgG antibody, and of anti-rat IgG antibody, and Alexa Fluor 488-donkey-anti-mouse IgG antibody were purchased from GE Healthcare, Cell LAB, and Invitrogen.
Honda T., Soeda S., Tsuda K., Yamaguchi C., Aoyama K., Morinaga T., Yuki R., Nakayama Y., Yamaguchi N, & Yamaguchi N. (2016). Protective role for lipid modifications of Src-family kinases against chromosome missegregation. Scientific Reports, 6, 38751.
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2

Protein Expression Analysis in Keratinocytes

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Total protein was extracted from keratinocytes in Leeds lysis buffer [56 (link)] and resolved by SDS-PAGE (10-15% Tris-Glycine), transferred onto Hybond nitrocellulose membrane (Amersham biosciences) and probed with antibodies specific for phosphorylated FRS2α (3861, Cell Signaling Technology), cyclin B1 (H-433, Santa Cruz Biotechnology), involucrin (SY5, Santa Cruz Biotechnology), HPV18 E6 (G-7, Santa Cruz Biotechnology), HPV18 E7 (8E2, Abcam (ab100953), AKT (9272, Cell Signaling Technology), phospho-AKT Ser473 (D9E, Cell Signaling Technology), HA (HA-7, Sigma H9658), cytokeratin 1 (Poly19056, Covance), phospho-ERK1/2 (43705, Cell Signalling Technology), GAPDH (G-9, Santa Cruz Biotechnology), phospho-GSK3α/β (9336, Cell Signalling Technology) and EGFR (R-1, Santa Cruz Biotechnology). Immunoblots were visualized with species-specific HRP conjugated secondary antibodies (Sigma) and ECL (Thermo/Pierce).
Wasson C.W., Morgan E.L., Müller M., Ross R.L., Hartley M., Roberts S, & Macdonald A. (2017). Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle. Oncotarget, 8(61), 103581-103600.
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3

Antibodies for Cellular Signaling Analysis

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The following antibodies were used: Fyn (#25; BD Biosciences, and Fyn3; Santa Cruz Biotechnology), c-Src (#327; Calbiochem), Src[pY416] (phospho-Src family, Cell Signaling Technology), γ-tubulin (GeneTex, and clone GTU-88; Sigma-Aldrich or Abcam), phosphotyrosine (pTyr) (4G10; Upstate Biotechnology, Inc.; a gift from T. Tamura and T. Yoshimoto), IAK1 (Aurora A) (#4; BD Biosciences), phospho-Aurora A(Thr288)/Aurora B(Thr232)/Aurora C(Thr198) (pAurora A/B/C) (D13A11; Cell Signaling Technology), HA (F7 and Y-11; Santa Cruz Biotechnology), myc (9E10; Santa Cruz Biotechnology and A-14-G; Santa Cruz Biotechnology), α-tubulin (MCA78G; Serotec), cyclin B1 (H-433; Santa Cruz Biotechnology), PRC1 (H-70; Santa Cruz Biotechnology), GM130 (#35; BD Transduction Laboratories), cPLA2 (4-4B-3C; Santa Cruz Biotechnology), LAMP-1 (H4A3; Santa Cruz Biotechnology), and PDI (P7496; Sigma). Horseradish peroxidase-linked IgG F(ab’)2 and Alexa Fluor 488-, 546-, or 647-labeled IgG secondary antibodies were obtained from Amersham Biosciences and Invitrogen.
Morii M., Kubota S., Hasegawa C., Takeda Y., Kometani S., Enomoto K., Suzuki T., Yanase S., Sato R., Akatsu A., Hirata K., Honda T., Kuga T., Tomonaga T., Nakayama Y., Yamaguchi N, & Yamaguchi N. (2021). Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle. Scientific Reports, 11, 2616.
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4

Paclitaxel-Resistant Breast Cancer Cells

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Parental T47D (T47DPAR) breast cancer cells were authenticated and provided by the Characterized Cell Line Core facility at The University of Texas MD Anderson Cancer Center (Houston, TX). T47DPAR and paclitaxel-resistant (T47DPAC) cells were cultured in phenol-red containing RPMI with 10% heat-inactivated fetal bovine serum (FBS), 100 IU/mL penicillin, and 100 μg/mL streptomycin. The cells were maintained at 37°C in a humidified incubator in an atmosphere of 5% CO2 and periodically tested for mycoplasma contamination. The antibodies used were as follows: p-pRb (Ser780) (#9307), pRb (#9309), p-NPM (Thr199) (#3541), p-JNK1/2 (p-Thr183/Tyr185) (#4671), JNK1 (2C6) (#3708), and JNK2 (#4672) purchased from Cell Signaling Technology; cyclin A (H-432) (#751), cyclin D1 (H-295) (#753), cyclin B1 (H-433) (#752), and p55CDC (H-175) (#8358) purchased from Santa Cruz Biotechnology; β-actin (AC-15) (#A1978) purchased from Sigma Aldrich; PCNA (#ab29) purchased from Abcam; and estrogen-receptor α (ERα) (SP1) (#RM-9101-S) purchased from Thermo Scientific Lab Vision.
Trapé A.P., Liu S., Cortes A.C., Ueno N.T, & Gonzalez-Angulo A.M. (2016). Effects of CDK4/6 Inhibition in Hormone Receptor-Positive/Human Epidermal Growth Factor Receptor 2-Negative Breast Cancer Cells with Acquired Resistance to Paclitaxel. Journal of Cancer, 7(8), 947-956.
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