Na k atpase kit

Berberine hydrochloride (HPLC ≥ 99%) and tetrahydropalmatine were purchased from Meilun Biologics (Dalian, China). Sodium bicarbonate was obtained from Macklin (Shanghai, China). Glutaraldehyde solution (50 wt% in H₂O) and glucose were purchased from Shanghai Titan Technology Co., Ltd. (Shanghai, China). Methanol at HPLC grade was purchased from Merck & Co., Inc. (Kenilworth, NJ). PBS (phosphate-buffered saline, 500 mL) and FITC (fluorescein isothiocyanate, 10 mg) were purchased from Tianjin Biolite Biotech Co, Ltd (Tianjin, China). All other chemicals were of analytical grade. NHC-LC-Biotin reagent was purchased from APExBIO Company (Houston, TX). FITC streptavidin was obtained from Nanjing Xinfan Biological Technology Co., Ltd. (Jiangsu, China) BCA Protein Quantification Kit was purchased from BOSTER Biological Technology, Wuhan, China. Na⁺/K⁺-ATPase Kit, Annexin V-FITC kit were both purchased from Nanjing Jiancheng Bioengineering Institute, Jiangsu, China.

The total protein of each gill sample was extracted using a Protein Extraction Kit (Invent, USA), and the protein concentration was then determined using a BCA Protein Assay Kit (Merck, GER) [7 (link)]. Subsequently, NKA activity was measured using a Na⁺-K⁺-ATPase Kit (Jiancheng, CHN) according to the manufacturer’s instructions [7 (link)], and H⁺-K⁺-ATPase activity was measured following the protocol supplied by the manufacturer of the H⁺-K⁺-ATPase Kit (JianCheng, CHN). ATP content assays were performed using an ATP assay kit (Jiancheng, CHN), following the manufacturer’s instructions.