Anti poly adp ribose polymerase

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-poly-ADP ribose polymerase is a laboratory reagent that recognizes and binds to poly-ADP ribose polymerase, a cellular enzyme involved in DNA repair processes.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti poly adp ribose polymerase

Shikonin Induces Oxidative Stress and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Shikonin was purchased from ChemFaces (Wuhan, China). Thiazolyl blue tetrazolium bromide (MTT), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), NAC, and other chemicals were obtained from Sigma-Aldrich (St. Louis, MI, USA). Anti-poly-ADP ribose polymerase was purchased from Cell Signaling Technology (No. 9542S, Beverly, MA, USA). Antibodies against ERK1/2 (ARG62350), phosphorylated ERK1/2 (ARG52277), and phosphorylated p38 (ARG51850) were obtained from Arigo Biolaboratories (Hsinchu City, Taiwan). Caspase 3 antibody (GTX110543) was purchased from GeneTex, Inc. (Hsinchu City, Taiwan). Light chain-3 (LC3)-II (NB 100-2220) antibody was obtained from Novus Biologicals (Centennial, CO, USA). Actin (6008-1-IG), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1-Ig), and p38 (66234-1-Ig) were obtained from Proteintech Group, Inc. (Rosemont, IL, USA). Bcl-2 (TGABF-188784-25) was obtained from TrustGene Biotech Company (Taichung, Taiwan).

Tsai M.F., Chen S.M., Ong A.Z., Chung Y.H., Chen P.N., Hsieh Y.H., Kang Y.T, & Hsu L.S. (2021). Shikonin Induced Program Cell Death through Generation of Reactive Oxygen Species in Renal Cancer Cells. Antioxidants, 10(11), 1831.

+ Open protocol

+ Expand

Reagents and Antibodies for Cell Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

PITC and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich; Merck KGaA. N-acetyl-L-cysteine (NAC) was purchased from Beyotime Institute of Biotechnology. PITC was dissolved in DMSO to prepare stock solutions for in vitro assays and stored at −20°C. Cells in the control groups were treated with vehicle at an equal volume. To minimize toxicity, DMSO concentration was >0.1% for cell culture. Anti-poly-ADP-ribose polymerase (PARP; catalog no. 9542; 1:1,000), anti-cleaved caspase-3 (catalog no. 9662; 1:1,000), anti-cleaved caspase-9 (catalog no. 9502; 1:1,000), anti-GAPDH (catalog no. 5174; 1:1,000), anti-cytochrome c (Cyt c; catalog no. 4272; 1:1,000), anti-p53 (catalog no. 2527; 1:1,000) and anti-phosphorylated p53 (p-p53; catalog no. 2521; 1:1,000) antibodies were purchased from Cell Signaling Technology, Inc. Anti-cyclin A1 antibody (catalog no. ab118897; 1:1,000) was obtained from Abcam, Inc. Antibodies against Bcl-2 (catalog no. A2845; 1:1,000), Bax (catalog no. A0207; 1:1,000) and β-tubulin (catalog no. AC008; 1:1,000 for western blot analysis and 1:400 for immunofluorescence assay) were purchased from ABclonal Biotech Co., Ltd.

Huang L., Cai C., Dang W., Lu J.H., Hu G.F, & Gu J. (2019). Propyl isothiocyanate induces apoptosis in gastric cancer cells by oxidative stress via glutathione depletion. Oncology Letters, 18(5), 5490-5498.

+ Open protocol

+ Expand

Calycopterin Anticancer Mechanisms in Vitro

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calycopterin, 5,4′-dihydroxy-3,6,7,8-tetramethoxyflavone was purified from D. kotschyi Boiss and the chemical structure was confirmed in our laboratory as reported previously [20 (link)]. MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide), dimethylsulfoximine (DMSO), and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA). Anti-cell division cycle 25 homolog C (cdc25c), anticyclin B1, anti-Bcl-, anti-Bax, anti-pro-caspase 3, anti-pro-caspase 9, anti-poly ADP-ribose polymerase (PARP), anti-extracellular signal-regulated kinase 1/2 (ERK1/2), anti-c-Jun N-terminal kinase (JNK), anti-p38 MAPK, anti-Akt, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-phospho-JNK (Thr183/Tyr185), anti-phospho-p38 MAPK (Thr180/Try182), anti-phospho-Akt (Ser473), and anti-phospho-phosphatidylinositol-3-kinase (PI3K) (Tyr458/Tyr199) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Benzyloxycarbonyl–Val–Ala–Asp–fluoromethyl ketone (z-VAD-fmk), LY294002, U0126, SP600125, and SB203580 were purchased from Promega (Madison, WI, USA). DMEM cell cultures, fetal bovine serum (FBS) were purchased from Gibco (Gaithersburg, MD, USA). All other reagents were commercial products of the highest available purity grade.

Esmaeili M.A., Farimani M.M, & Kiaei M. (2014). Anticancer effect of calycopterin via PI3K/Akt and MAPK signaling pathways, ROS-mediated pathway and mitochondrial dysfunction in hepatoblastoma cancer (HepG2) cells. Molecular and cellular biochemistry, 397(1-2), 17-31.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!