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Mil 36γ

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MIL-36γ is a laboratory equipment product that serves a core function. However, a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

Recombinant mil 1β, by R&D Systems (2 mentions) Recombinant hil 1β, by R&D Systems (2 mentions) Mil 33, by R&D Systems (2 mentions) Steady glo, by Promega (2 mentions) M1000 plate reader, by Tecan (2 mentions)

2 protocols using mil 36γ

1

Antagonism of IL-1, IL-33, and IL-36 Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The functionality of MAB-mR3 was tested in murine NIH-3T3 (NF-κB) Luciferase reporter cell lines (Signosis, DMEM, 10% FCS). Seeded in 384-well flat bottom plates (20,000 cells/well (30,000 cells/well for IL-33 stimulations)) and rested for 16hrs (37°C/5% CO2) before pre-incubating 1hr with MAB-mR3 antibody at increasing concentrations. Recombinant mIL-1β (50pg/mL), mIL-33 (1ng/mL) or mIL-36γ (170ng/mL) (R&D Systems) was added and plates incubated for 5hrs (37°C/5% CO2). Steady-Glo™ (Promega) solution was used on lysates and luminescence measured (Tecan M1000 plate reader). Inhibition of IL-6 release was determined in a NIH-3T3 cell line (ATCC). Seeded in 384-well flat bottom plates (12,500 cells/well) and rested for 2hrs (37°C/5% CO2) before pre-incubating 1hr with MAB-mR3 or MAB-hR3 antibody at increasing concentrations. Cells were stimulated with recombinant hIL-1β (50pg/mL, R&D Systems) for 16hrs and assayed for IL-6 production.
Højen J.F., Vindvad Kristensen M.L., McKee A.S., Wade M.T., Azam T., Lunding L.P., de Graaf D.M., Swartzwelter B.J., Wegmann M., Tolstrup M., Beckman K., Fujita M., Fischer S, & Dinarello C.A. (2019). IL-1R3 blockade broadly attenuates the functions of six members of the IL-1 family, revealing their contribution to models of disease. Nature immunology, 20(9), 1138-1149.
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2

Antagonism of IL-1, IL-33, and IL-36 Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The functionality of MAB-mR3 was tested in murine NIH-3T3 (NF-κB) Luciferase reporter cell lines (Signosis, DMEM, 10% FCS). Seeded in 384-well flat bottom plates (20,000 cells/well (30,000 cells/well for IL-33 stimulations)) and rested for 16hrs (37°C/5% CO2) before pre-incubating 1hr with MAB-mR3 antibody at increasing concentrations. Recombinant mIL-1β (50pg/mL), mIL-33 (1ng/mL) or mIL-36γ (170ng/mL) (R&D Systems) was added and plates incubated for 5hrs (37°C/5% CO2). Steady-Glo™ (Promega) solution was used on lysates and luminescence measured (Tecan M1000 plate reader). Inhibition of IL-6 release was determined in a NIH-3T3 cell line (ATCC). Seeded in 384-well flat bottom plates (12,500 cells/well) and rested for 2hrs (37°C/5% CO2) before pre-incubating 1hr with MAB-mR3 or MAB-hR3 antibody at increasing concentrations. Cells were stimulated with recombinant hIL-1β (50pg/mL, R&D Systems) for 16hrs and assayed for IL-6 production.
Højen J.F., Vindvad Kristensen M.L., McKee A.S., Wade M.T., Azam T., Lunding L.P., de Graaf D.M., Swartzwelter B.J., Wegmann M., Tolstrup M., Beckman K., Fujita M., Fischer S, & Dinarello C.A. (2019). IL-1R3 blockade broadly attenuates the functions of six members of the IL-1 family, revealing their contribution to models of disease. Nature immunology, 20(9), 1138-1149.
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