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Igd pe cy7

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The IgD PE-Cy7 is a fluorochrome-conjugated antibody that binds to the IgD protein. It is designed for use in flow cytometry applications to detect and analyze IgD-expressing cells.

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Lab products found in correlation

Cd21 apc, by BioLegend (3 mentions) Cd19 fitc, by BioLegend (3 mentions) Facscanto 2, by BD (3 mentions) Ki67 fitc, by BD (2 mentions) Apc fire750 cd27, by BioLegend (2 mentions) Percp cy5.5 cxcr5, by BioLegend (2 mentions) Percp cy5.5 igg, by BioLegend (2 mentions) Hla dr apc, by BioLegend (2 mentions) Pe cy5 cd69, by BioLegend (2 mentions) Pe cy5 cd86, by BioLegend (2 mentions) Cd80 allophycocyanin apc, by BD (1 mentions) Cd45ro pe cy7, by BD (1 mentions) Ifn γ fitc, by BD (1 mentions) Pd 1 pe, by BD (1 mentions) Isotype control abs, by BD (1 mentions) Guava 8ht flow cytometer, by Merck Group (1 mentions) Cd40l pe, by BD (1 mentions) Cd27 apc cy7, by BD (1 mentions) Cd38 apc, by BD (1 mentions) Annexin 5 fitc, by BD (1 mentions)

Close spelling variants of this product

Anti-IgD-PE-Cy7 (4 citations) IgD-PE (7 citations)

7 protocols using igd pe cy7

1

Multicolor Flow Cytometry Analysis

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The fluorochrome-labeled monoclonal antibodies used in this study included: antibodies against CD3-Peridinin Chlorophyll (Percp, Miltenyi Biotec), CD8-APCcy (Biolegend), CD4-phycoerytherin (PE, BD Pharmingen), CD27-APCcy7 (BD Pharmingen), IgD-PEcy7 (Biolegend, San Diego, CA), CD40L-PE (BD Pharmingen), CD19- fluorescein isothiocyanate (FITC, BD Pharmingen), CD80-allophycocyanin (APC, BD Pharmingen), PD1-PE (BD Pharmingen), CD45RO-PEcy7 (BD Pharmingen), ki67-FITC (BD Pharmingen), IFN-γ-FITC (BD Pharmingen), CD38-APC, annexin V-FITC, and isotype control Abs (BD Pharmingen). No annexin V staining was used as a control for gating strategy. All others were gated based on isotypes. Cells were identified by their forward (FSC) and side scatter (SSC) characteristics and were analyzed with a Guava 8HT flow cytometer (Millipore, Billerica, MA).
Powell A.M., Luo Z., Martin L., Wan Z., Ma L., Liao G., Song Y., Li X., Kilby J.M., Huang L, & Jiang W. (2016). The induction of CD80 and apoptosis on B cells and CD40L in CD4+ T cells in response to seasonal influenza vaccination distinguishes responders versus non-responders in healthy controls and aviremic ART-treated HIV-infected individuals. Vaccine, 35(5), 831-841.
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2

Multiparameter Flow Cytometry Assay

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The following anti-human Abs were used in this study: 7-AAD PerCP, CD8 PE, CD16 APC-Cy7, CD21 APC, CD38 PE, CD56 PE-Cy7, DNAM-1 FITC, GM-CSF PerCP/Cy5.5, IFN-γ APC, Ki 67 FITC (all BD Biosciences); CD3 Pacific Blue (Life Technologies); CD4 APC, CD19 PE, (eBioscience); CD20 PE-Cy5, CD27 APC-Cy7, IgD PE-Cy7, NKp44 APC (BioLegend); CD158a, h PE-Cy5.5, CD158b1/b2, j PE-Cy5.5, CD158a, h APC, CD158b1/b2, j APC, CD159a (NKG2A) PE, CD159a (NKG2A) APC (Beckmann Coulter); hIL-12 Rß PerCP (R&D Systems). Live cells were distinguished using the LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies). Following endotoxin free recombinant human (rh) cytokines were used: rhIL-12 (R&D Systems), rhIL-2 (PeproTech), and rhIL-15 (Sigma).
Jud A., Kotur M., Berger C., Gysin C., Nadal D, & Lünemann A. (2016). Tonsillar CD56brightNKG2A+ NK cells restrict primary Epstein-Barr virus infection in B cells via IFN-γ. Oncotarget, 8(4), 6130-6141.
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3

Characterization of Lymph Node Immune Cells

Cited 1 time
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Mandibular lymph nodes were macerated over 40-μm mesh cell strainer (Midsci, Valley Park, MO) into single-cell suspensions. Cells were enumerated and 1 × 106 cells/100 μl PBS containing 2% FBS were blocked with anti-CD16/32 (eBioscience, San Diego, CA), labeled with a combination of 1 μl each of CD45 PerCP-Cy5.5 (Biolegend, San Diego, CA), CD19 APC, CD3e, CD4 APC-Cy7, CD8a PE or APC-Cy7, IgM FITC, IgD PE-Cy7, CD44 APC, and/or CD62L FITC (all from Thermo Fischer Scientific) diluted in 1% BSA in 1X PBS for 30 minutes. In the case of tetramer staining, cells were labeled with a combination of CD3 PE-Cy7, CD8-APC-Cy7, gB (SSIEFARL)-PE or ICP6 (QTFDFGRL)-Alexafluor488 (NIH Tetramer Core Facility, Atlanta, GA). Cells were then washed twice by adding 1 ml of 2% FBS in 1X PBS, centrifuging for 5 minutes at 300 × g, and decanting supernatant. Cells were then fixed in 1 ml of 1% paraformaldehyde overnight and resuspended in 1 ml of 2% FBS in 1X PBS to be analyzed on a MacsQuant 196 flow cytometer (Miltenyi Biotech). Gating strategies were identical to those previously described [40 (link)] except those included in this study, incubated on ice in the dark for 20–30 min, and washed in PBS containing 2% FBS. Samples were analyzed using FlowJo software (Ashland, OR).
Carr D.J., Berube A.N., Filiberti A, & Gmyrek G.B. (2021). Lack of Neonatal Fc Receptor does not Diminish the Efficacy of the HSV-1 0ΔNLS Vaccine Against Ocular HSV-1 Challenge. Vaccine, 39(18), 2526-2536.
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4

Comprehensive Immune Cell Phenotyping

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PBMC (1–4×106 cells/tube) were stained with monoclonal antibodies to B cell markers (CD19-AF700, IgM-PerCP-Cy5.5, IgD-PE-Cy7 (Biolegend), CD38-FITC, CD21-PE, CD10-PE-CF594, CD40-APC, CD86-BV421 (BD Pharmingen)) and T cell markers (CD3-AF700, CD4PacBlue (Biolegend), CD8-APC-AF750 (Invitrogen), CD45RA-ECD, CD27-PE-Cy5 (Beckman Coulter), CD27-BV650, CD38-FITC, HLA-DR-PE-Cy7, PD-1-APC, CXCR5-PE (R&D Systems)) for 40 minutes at room temperature. Stained cells were subsequently washed twice and fixed in 1% paraformaldehyde for 10 minutes at 4°C. Data was acquired within 4 hours using a BD LSRII flow cytometer (BD Biosciences – Immunocytometry Systems, San Jose, CA). Data were analyzed using Flow Jo Software (Tree Star Inc., Ashland, OR) with the gating scheme as per Supplemental Figure 1.
Nicholson L.K., Pratap H., Bowers E., Gunzburger E., Bandi S., Gardner E.M., Palmer B.E., Wright T., Kittelson J, & Janoff E.N. (2018). Effective B cell Activation in vitro during Viremic HIV-1 Infection with Surrogate T cell Stimulation. Immunobiology, 223(12), 839-849.
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5

Acute Malaria PBMC Immunophenotyping

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PBMCs collected during acute malaria were used for MBC subset and plasmablast/plasma cell phenotyping. Fluorochrome-conjugated, mouse anti-human monoclonal antibodies were used to stain 1 million PBMCs/100 μL FACS buffer. A cocktail consisting of the following mouse monoclonal antibodies was used: FITC-CD19, APC-CD21, APC/fire-CD27, AF700-CD38, PE-IgM and PE/Cy7-IgD (Biolegend, San Diego, CA, USA). After staining for 15 min, cells were washed with FACS buffer. Finally, cells were suspended in 250 μL FACS buffer. The analyses were done with a flow cytometer (FACSCanto II, Becton–Dickinson Immunocytometry Systems, San Jose, CA, USA). Data were processed using FlowJo software (Tree Star, San Carlos, CA, USA).
Kochayoo P., Sanguansuttikul P., Thawornpan P., Wangriatisak K., Adams J.H., Ntumngia F.B, & Chootong P. (2021). The presence of circulating antibody secreting cells and long-lived memory B cell responses to reticulocyte binding protein 1a in Plasmodium vivax patients. Malaria Journal, 20, 474.
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6

Characterizing B Cell Subsets in SLE

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To detect subpopulations and characterize phenotypes of B cells, PBMCs from SLE patients (n = 20) were separated and analyzed to determine the frequency of each B cell subset. Two × 105 cells were resuspended in 50 μl FACS buffer and incubated with surface antibodies for 15 min at 4 °C. Antibodies used included FITC-CD19, APC-CD21, APC/Fire750-CD27, Alexa/fluorro700-CD11c, PerCP/Cy5.5-CXCR5, PerCP/Cy5.5-IgG, PE/Cy7-IgD, APC-HLA-DR, PE/Cy5-CD69, and PE/Cy5-CD86 (Biolegend, San Diego, USA). After staining, cells were washed with 1 ml FACS buffer and resuspended in 300 μl FACS buffer for surface marker analyses.
The frequency and phenotype of DNA tetramer-binding B cells were detected by the tetramer staining technique, resuspended in 100 μl FACS buffer, and incubated with surface antibodies for 15 min at 4 °C. The stained B cells were washed with 1 ml FACS buffer and resuspended in 500 μl FACS buffer for flow cytometric surface marker analysis. FACS data were acquired with a BD FACS Canto II (Becton-Dickinson Immunocytometry Systems, San Jose, USA) and analyzed with FlowJo software (v. 10.0; Tree Star Inc., CA, USA).
Wangriatisak K., Thanadetsuntorn C., Krittayapoositpot T., Leepiyasakulchai C., Suangtamai T., Ngamjanyaporn P., Khowawisetsut L., Khaenam P., Setthaudom C., Pisitkun P, & Chootong P. (2021). The expansion of activated naive DNA autoreactive B cells and its association with disease activity in systemic lupus erythematosus patients. Arthritis Research & Therapy, 23, 179.
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7

Phenotypic Profiling of B Cells in SLE

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To detect subpopulations and characterize phenotypes of B cells, PBMCs from SLE patients (n = 20) were separated and analyzed to determine the frequency of each B cell subset. Two x 10 5 cells were resuspended in 50 µl FACS buffer and incubated with surface antibodies for 15 min at 4 o C. Antibodies used included FITC-CD19, APC-CD21, APC/Fire750-CD27, Alexa/ uorro700-CD11c, PerCP/Cy5.5-CXCR5, PerCP/Cy5.5-IgG, PE/Cy7-IgD, APC-HLA-DR, PE/Cy5-CD69, and PE/Cy5-CD86 (Biolegend, San Diego, USA). After staining, cells were washed with 1 ml FACS buffer and resuspended in 300 µl FACS buffer for surface marker analyses.
The frequency and phenotype of DNA tetramer-binding B cells were detected by the tetramer staining technique, resuspended in 100 µl FACS buffer, and incubated with surface antibodies for 15 min at 4 o C.
The stained B cells were washed with 1 ml FACS buffer and resuspended in 500 µl FACS buffer for ow cytometric surface marker analysis. FACS data were acquired with a BD FACS Canto II (Becton-Dickinson Immunocytometry Systems, San Jose, USA) and analyzed with FlowJo software (v. 10.0; Tree Star Inc., CA, USA).
Wangriatisak K., Thanadetsuntorn C., Krittayapoositpot T., Leepiyasakulchai C., Suangtamai T., Ngamjanyaporn P., Khowawisetsut L., Khaenam P., Setthaudom C., Pisitkun P., & Chootong P. (2021). The expansion of activated naive DNA autoreactive B Cells and its association with disease activity in Systemic Lupus Erythematosus patients.
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