C peptide

Manufactured by Crystal Chem

Sourced in Sweden

C-peptide is a laboratory equipment used for the detection and measurement of C-peptide, a byproduct of insulin production in the body. It serves as an indicator of insulin secretion and can be used to assess pancreatic beta-cell function.

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Lab products found in correlation

Insulin, by Crystal Chem (3 mentions) Ab269542, by Abcam (1 mentions) Ab197742, by Abcam (1 mentions) Fgf19, by R&D Systems (1 mentions) Glp 1, by Abcam (1 mentions) Il 1β, by Abcam (1 mentions) 7180 clinical analyzer, by Hitachi (1 mentions) Interleukin 6 (il 6), by Mesoscale (1 mentions) Hemoglobin a1c, by Crystal Chem (1 mentions) Adiponectin, by Crystal Chem (1 mentions) Ezrmfgf21 26k, by Merck Group (1 mentions) Fasting insulin, by Mercodia (1 mentions) Humulin, by Novo Nordisk (1 mentions) Proinsulin, by Mercodia (1 mentions) Glucagon, by Crystal Chem (1 mentions) Insulin, by Mercodia (1 mentions)

Close spelling variants of this product

C-peptide ELISA Mouse C-Peptide ELISA Kit Mouse C-peptide ELISA

7 protocols using c peptide

Metabolic Biomarker Profiling in Diabetes

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In the cohort study, participants’ blood HbA1c, FBG, C-peptide, WBC, NEUT, LYMPH, TC, TG, HDL-C, and LDL-C levels were measured according to uniform national quality control protocols. Serum GLP-1 (Abcam, Cambridge, UK; ab184857), FGF19 (R&D Systems, Minneapolis, MN, USA; DF1900), LBP (Panchao Biotechnology, Shanghai, China; PCDBH0284), and IL-1β (Panchao Biotechnology, PCDBH0247) concentrations were measured using enzyme-linked immunosorbent assay kits, according to the manufacturer’s instructions.
In the animal experiments, HbA1c was determined by an enzymatic method using a Hitachi 7180 Clinical Analyzer (Hitachi, Tokyo, Japan). Serum insulin (Crystal Chem Inc., 90080), C-peptide (Crystal Chem Inc., 90050), IL-1β (Abcam, ab197742), and LBP (Abcam, ab269542) concentrations were measured using the mouse ELISA kits as per the manufacturer’s instructions.

Yuan X., Wang R., Han B., Sun C., Chen R., Wei H., Chen L., Du H., Li G., Yang Y., Chen X., Cui L., Xu Z., Fu J., Wu J., Gu W., Chen Z., Fang X., Yang H., Su Z., Wu J., Li Q., Zhang M., Zhou Y., Zhang L., Ji G, & Luo F. (2022). Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes. Nature Communications, 13, 6356.

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Colorimetric Biomarker Quantification Protocol

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Colorimetric reagents were used to measure the following analytes: glucose (Cat #997-03001, Wako Scientific, Richmond, VA, United States), non-esterified fatty acids (NEFA) (Wako Scientific NEFA-HR, Richmond, VA, United States), triglycerides, and total cholesterol (Cat #T7532 and #C7510, Pointe Scientific, Canton, MI). The following analytes were determined by ELISA: IL-6 (Cat #K15069L-1, Meso Scale Discovery, Rockville, MD, United States), insulin, and C-peptide (Cat #90080 and #90050, Crystal Chem, Elk Grove Village, IL, United States). Homeostatic Model Assessment for insulin Resistance (HOMA-IR) was calculated from fasting glucose and insulin values using the equation HOMA-IR = Fasting plasma glucose (mg/dl) × Fasting plasma insulin (ng/ml)/18.05 (Matthews et al., 1985 (link)).

Schwartzkopf C.D., Hadcock J.R., Liu G., Germano P., Roux J., Shea C.M., Buys E.S, & Jones J.E. (2022). Beneficial Metabolic Effects of Praliciguat, a Soluble Guanylate Cyclase Stimulator, in a Mouse Diet-Induced Obesity Model. Frontiers in Pharmacology, 13, 852080.

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Comprehensive Metabolic Profiling of Mice

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Commercially-available colorimetric assay kits were used to measure the activity and/or concentration in whole blood or blood plasma of each of the following: Hemoglobin A1c (Catalog #80,310, Crystal Chem, Elk Grove Village); alanine aminotransferase (ALT) activity (Cat#MAK052, MilliporeSigma, St. Louis);, aspartate aminotransferase (AST) activity (Cat#MAK055, MilliporeSigma), triglycerides (Cat#100,103,003, Cayman Chemical), and urea concentration (Cat#MAK006). Plasma adiponectin (Cat#80,569), C-Peptide (Cat#90,050), insulin (Cat#90,080), and leptin (Cat#90,030) concentrations were assessed by ELISA (Crystal Chem). All samples were measured in triplicate and samples from a minimum of three mice were measured per study timepoint.

Phillips B.E., Lantier L., Engman C., Garciafigueroa Y., Singhi A., Trucco M., Mantzoros C., Wasserman D, & Giannoukakis N. (2022). Improvement in insulin sensitivity and prevention of high fat diet-induced liver pathology using a CXCR2 antagonist. Cardiovascular Diabetology, 21, 130.

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Measuring Plasma C-peptide, FGF21, and GLP-1

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C-peptide (Crystal Chem, #90050) and FGF21 (EZRMFGF21–26 K; Millipore, Billerica, MA) were analyzed individually by ELISA assay and total GLP-1 was determined using the Meso Scale Assay System (Cat# K150JVC, Mesoscale Discovery, Rockville, MD) in EDTA-collected plasma.

Rivero-Gutierrez B., Haller A., Holland J., Yates E., Khrisna R., Habegger K., Dimarchi R., D'Alessio D, & Perez-Tilve D. (2018). Deletion of the glucagon receptor gene before and after experimental diabetes reveals differential protection from hyperglycemia. Molecular Metabolism, 17, 28-38.

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Glucose Homeostasis Assessment in Mice

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Oral glucose tolerance tests (OGTTs) were performed after at least a 3-day resting period following the MR session (described below). Six-hour fasted mice (food removed at ~07:30 h) received a glucose load of 1.5 g/kg by oral gavage, and blood glucose was monitored from the tail tip with a glucometer (Breeze, Bayer, Zürich, Switzerland) for 2 h. The area under the curve (AUC) in the glucose versus time plot was calculated to assess glucose clearance from the blood. An insulin tolerance test (ITT) was then performed after a minimum 3-day resting period by intraperitoneal injection of insulin (0.75 IU/kg, Humulin, Novo Nordisk, Bagsvaerd, Denmark, diluted in 0.9% NaCl). Blood glucose was monitored from the tail tip for 3 h and the area above the curve (AAC) was calculated for the glucose versus time plots. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to measure fasting insulin (Mercodia, Uppsala, Sweden) and C-peptide (Crystal Chem, Downers Grove, IL, USA) levels in the plasma. Insulin sensitivity was estimated with the quantitative insulin sensitivity check index (QUICKI) as the inverse of the log 10 sum of fasting insulin (μIU/mL) and fasting glucose (mg/dL).

Soares A.F., Paz-Montoya J., Lei H., Moniatte M, & Gruetter R. (2017). Sexual dimorphism in hepatic lipids is associated with the evolution of metabolic status in mice. NMR in biomedicine, 30(10).

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Multiplex Cytokine and SCFA Analysis

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Plasma cytokine/chemokine levels (eotaxin, CCL5, CXCL10, G-CSF, GM-CSF, Fractalkine, IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IL-18, MCP-1, MIP-1a, RANTES, TGFB1, TGFB2, TGFB3, TNFa, VEGF) were measured by 30-plex ELISA (Eve Technologies, Calgary, AB. Canada). Circulating SCFA levels (acetate, propionate, isobutyrate, butyrate, isovalerate, valerate, isocaproate, hexanoate) were measured by mass spectrometry at the Mayo Clinic Metabolomics Research Core as described.^{33 (link)} Commercial ELISAs were used to quantify non-fasting proinsulin (Mercodia, Uppsala, Sweden) and C-peptide (Crystal Chem, Elk Grove, IL, USA) in plasma.

Sargin P., Roethle M.F., Jia S., Pant T., Ciecko A.E., Atkinson S.N., Salzman N.H., Teng R.J., Chen Y.G., Cabrera S.M, & Hessner M.J. (2022). Lactiplantibacillus plantarum 299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats. Gut Microbes, 14(1), 2136467.

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Fasting Biomarkers During OGTT

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Fasting serum samples collected during OGTT, pre-training and at least 48 hours after the last exercise bout of the training program, were analyzed for insulin (Mercodia), C-peptide (Crystal Chem), amylin (Novus), glucagon (Crystal Chem), and pro-insulin (Crystal Chem) only at 0 min of OGTT, according to manufacturer’s instructions. RREB1 was measured in fasting serum samples before, immediately after, and 30minutes of rest post VO2_peak using a commercially available ELISA (Mybiosource). All the assays were run in duplicates.

Zhang H., Simpson L.K., Carbone N.P., Hirshman M.F., Nigro P., Vamvini M., Goodyear L.J, & Middelbeek R.J. (2023). Moderate-intensity endurance training improves late phase β-cell function in adults with type 2 diabetes. iScience, 26(7), 107226.

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