Tubulin clone dm1a

Cell extracts were prepared in RIPA lysis buffer (NEB) supplemented with protease inhibitors (Sigma) and phosphatase inhibitors (Sigma, NEB). Immunoblots were performed using standard procedures. Protein samples were separated by SDS–PAGE (4–12% gradient gels; Invitrogen) and subsequently transferred onto nitrocellulose membranes. All primary antibodies were used at 1:1000 dilution with the exception of BLM (1:500) and RMI1 (1:5000). Secondary antibodies were used at 1:5000. The following antibodies were used: FANCC clone 8F3 (Merck Millipore), FANCD2 EPR2302 (Abcam), FANCI A301-254 (Bethyl laboratories), NQO1 clone A180 (Cell Signaling), RMI1 (Proteintech), BLM clone C-18 (Santa Cruz), MUS81 clone MTA30 2G10/3 (Abcam), p21 clone F-5 (Santa Cruz), β-Actin clone 20–33 (Sigma), Tubulin clone DM1A (Cell Signaling), HRP-conjugated goat anti-mouse, rabbit or goat IgG (Jackson Immunochemicals). The p53 antibody (PAB 421) was from Cancer Research UK. Uncropped immunoblot images are shown in Supplementary Fig. 7.

Kasumi-1 and SKNO-1 cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany), respectively. Cell were maintained in RPMI-1640 media supplemented with 20% FBS. SKNO-1 cells were also supplemented with 10ng/ml recombinant GM-CSF. Following antibodies were used in this study: RUNX1 (Cat. No. 4334, Cell Signaling Technology, Inc., Danvers, MA), ETO (Cat. No. 4498S, Cell Signaling Technology, Inc.), RUNX1T1/ETO (Cat. No. ab124269; Abcam, Cambridge, MA), CDK6 Clone DCS83 (Cat. No. 3136S; Cell Signaling Technology, Inc.), Tubulin Clone DM1A (Cat. No. T9026; Sigma-Aldrich, St. Louis, MO), and normal (Cat. No. sc-2027 for anti-rabbit; Cat. No. sc-2025 for anti-mouse) and HRP-conjugated IgG (Cat. No. sc-2004 for anti-rabbit; Cat. No. sc-2005 for anti-mouse) antibodies (Santa Cruz Biotechnology, Dallas, TX).