Human CRC cell lines (colo-201, colo-205, colo-320, HCT-15, HCT-116, HT-29, Lovo, LS174T, SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-977, SNU-1033, SNU-1181, SNU-1235, SNU-1411, SNU-1544, SNU-1684, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, SW-403, and SW-480) were purchased from the Korean Cell Line Bank [18 (link)]. All cell lines were cultured in RPMI1640 (Hyclone Laboratories, Ind., Logan, UT), supplemented with 10% fetal bovine serum, at 37°C in humidified 5% CO2.
Snu 61
Manufactured by Korean Cell Line Bank
The SNU-61 is a cell culture incubator designed for maintaining and growing cell lines. It provides a controlled environment with regulated temperature, humidity, and CO2 levels to support optimal cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Snu 407, by Korean Cell Line Bank (2 mentions)
Snu c4, by Korean Cell Line Bank (1 mentions)
Snu 175, by Korean Cell Line Bank (1 mentions)
Hct116, by Korean Cell Line Bank (1 mentions)
Sw480, by Korean Cell Line Bank (1 mentions)
Colo205, by Korean Cell Line Bank (1 mentions)
Ls174t, by Korean Cell Line Bank (1 mentions)
Snu c1, by Korean Cell Line Bank (1 mentions)
Hct 15, by Korean Cell Line Bank (1 mentions)
Sw403, by Korean Cell Line Bank (1 mentions)
Snu 81, by Korean Cell Line Bank (1 mentions)
Colo201, by Korean Cell Line Bank (1 mentions)
Colo 320, by Korean Cell Line Bank (1 mentions)
Snu 70, by Korean Cell Line Bank (1 mentions)
Snu 283, by Korean Cell Line Bank (1 mentions)
Ht 29, by Korean Cell Line Bank (1 mentions)
Rpmi 1640, by Cytiva (1 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
2 protocols using snu 61
1
Culturing Human CRC Cell Lines
2
Culturing and Differentiating Caco-2 and Human IECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human epithelial cell line, Caco-2, was obtained from the ATCC. The cells were maintained in complete Dulbecco's modified Eagle medium (DMEM; Welgene, Daegu, Republic of Korea) with 10% fetal bovine serum (FBS; GIBCO, Burlington, ON, Canada) and 1% penicillin-streptomycin (Hyclone, Logan, UT, USA) at 37℃ in a humidified 5% CO 2 incubator. For differentiation/polarization, Caco-2 cells were plated on 12-mm transwell inserts with a 0.4μm pore polycarbonate membrane (Costar, Corning, NY, USA) and incubated for up to 21 days. The differentiation/ polarization was confirmed by measuring the trans-epithelial electrical resistance (TEER) at > 400 Ω•cm 2 with an EVOM2 (World Precision Instruments, Sarasota, FL, USA). Primary human IECs, SNU-61 and SNU-407 cells, were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea) and cultured in complete Roswell Park Memorial Institute-1640 medium (Welgene) containing 10% FBS and 1% penicillin-streptomycin at 37℃ in a humidified CO 2 incubator.
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