Sc 9757

Antibodies against ADAM12 were as previously described [23 (link),36 (link),37 (link)]. The rabbit polyclonal antibodies N-19 (sc-9752) and H-200 (sc-13976) against BSG, and goat polyclonal antibody L-19 against PLAP (sc-9757) were from Santa Cruz Biotechnology (Dallas, TX, USA). Other antibodies used were rabbit polyclonal antibody PA5-29787 against BSG (Thermo Fischer Scientific, Waltham, MA, USA), mouse monoclonal antibodies against actin (MAB1501, Millipore Chemicon, Burlington, MA, USA) and FITC-conjugated anti-CD147 (555962, BD Biosciences, San Jose, CA, USA). Living Colors^® GFP Monoclonal Antibody from Clontech was used for co-immunoprecipitation studies. Secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-mouse, goat anti-rabbit, and rabbit anti-goat immunoglobulins from DAKO A/S (Glostrup, Denmark). Alexa Fluor 488-conjugated rabbit anti-goat IgG and goat anti-mouse IgG, and Alexa Fluor 546-conjugated goat anti-mouse IgG F(ab)₂ fragment and goat anti-rabbit IgG F(ab)₂ fragment were from Invitrogen (Taastrup, Denmark). The metalloprotease inhibitors Batimastat, GM6001, and all other chemicals were from Merck KGaA (Darmstadt, Germany).

A rat monoclonal antibody against mouse SOF1 (amino acid #22-#89, ENSMUST00000031900) was generated as described in SI Appendix. Monoclonal antibodies for SLC2A3 (KS64-10), IZUMO1 (KS64-125), and ADAM1B (KS107-158) were generated in our laboratory (46 (link), 47 (link)). The anti-1D4 antibody was generated using a hybridoma cell line as a gift from Robert Molday, Ophthalmology and Visual Sciences, Centre for Macular Research, University of British Columbia, Vancouver, British Columbia, Canada (48 (link)). A polyclonal antibody for BASIGIN was purchased from Santa Cruz Biotechnology (sc-9757). A monoclonal antibody against β-Actin (ACTB) was purchased from Abcam (ab6276). A monoclonal and a polyclonal antibody against FLAG were purchased from Sigma Aldrich (F1804) and MBL (PM020), respectively. Horseradish peroxidase (HRP)-conjugated goat anti-rat immunoglobulins (IgGs) (112-035-167), HRP-conjugated goat anti-mouse IgGs (115-036-062), and HRP-conjugated bovine anti-goat IgGs antibodies (805-035-180) were purchased from Jackson ImmunoResearch Laboratories. Fluorophore-conjugated secondary antibodies goat-anti rat IgG Alexa Fluor 488 (A11006), goat-anti rat IgG Alexa Fluor 546 (A11081), goat-anti mouse IgG Alexa Fluor 488 (A11017), and goat-anti mouse IgG Alexa Fluor 546 (A11018) were purchased from Thermo Fisher Scientific.