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Anti total egfr

Manufactured by Merck Group
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Anti-total EGFR is a laboratory reagent used for the detection and quantification of total epidermal growth factor receptor (EGFR) protein in biological samples. It is designed to provide accurate and reliable measurements of EGFR levels, which are important in various research and diagnostic applications.

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Lab products found in correlation

Anti β actin, by Merck Group (2 mentions) Anti phospho egfr tyr1068, by Cell Signaling Technology (2 mentions) Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Anti aid, by Thermo Fisher Scientific (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Anti phospho ser473 p akt, by Cell Signaling Technology (2 mentions) Anti april, by Thermo Fisher Scientific (2 mentions) Anti fibrillrin, by Santa Cruz Biotechnology (2 mentions) Anti p65, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Anti-EGFR (21 citations)

2 protocols using anti total egfr

1

Quantitative Western Blot Analysis of Immune Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
MSIE cells and intestinal epithelial cells isolated from mice were solubilized in cell lysis buffer to prepare total cellular lysates. Nuclear fractions were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) according to the manufacturer’s instructions. The protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific Inc.).
Cellular and nuclear lysates were mixed with Laemmli sample buffer and proteins were separated by SDS-PAGE for Western blot analysis using anti-phospho-Ser473(P)-Akt (Cell Signalling Danvers, MA), anti-phospho-Tyr1068-EGFR (Cell Signalling), anti-total EGFR (Millipore, Billerica, MA), anti-APRIL (Thermo scientific), anti-BAFF (Enzo Biochem, Inc, Farmingdale, NY), anti-AID (eBioscience), anti-p65 (Santa Cruze, Dallas, TX), anti-fibrillrin (Santa Cruze), and anti-β actin (Sigma-Aldrich) antibodies.
Wang Y., Liu L., Moore D.J., Shen X., Peek RM J.r., Acra S.A., Li H., Ren X., Polk D.B, & Yan F. (2016). A LGG-derived protein promotes IgA production through up-regulation of APRIL expression in intestinal epithelial cells. Mucosal immunology, 10(2), 373-384.
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2

Quantitative Western Blot Analysis of Immune Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
MSIE cells and intestinal epithelial cells isolated from mice were solubilized in cell lysis buffer to prepare total cellular lysates. Nuclear fractions were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific) according to the manufacturer’s instructions. The protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific Inc.).
Cellular and nuclear lysates were mixed with Laemmli sample buffer and proteins were separated by SDS-PAGE for Western blot analysis using anti-phospho-Ser473(P)-Akt (Cell Signalling Danvers, MA), anti-phospho-Tyr1068-EGFR (Cell Signalling), anti-total EGFR (Millipore, Billerica, MA), anti-APRIL (Thermo scientific), anti-BAFF (Enzo Biochem, Inc, Farmingdale, NY), anti-AID (eBioscience), anti-p65 (Santa Cruze, Dallas, TX), anti-fibrillrin (Santa Cruze), and anti-β actin (Sigma-Aldrich) antibodies.
Wang Y., Liu L., Moore D.J., Shen X., Peek RM J.r., Acra S.A., Li H., Ren X., Polk D.B, & Yan F. (2016). A LGG-derived protein promotes IgA production through up-regulation of APRIL expression in intestinal epithelial cells. Mucosal immunology, 10(2), 373-384.
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