Applications suite software

Manufactured by Leica

Sourced in United States, China

The Leica Applications Suite is a software package that provides a comprehensive solution for various microscopy applications. It offers a user-friendly interface and a range of tools for image acquisition, analysis, and management. The software supports a variety of Leica microscopes and imaging systems, allowing users to acquire, process, and analyze high-quality images with ease.

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Lab products found in correlation

Phospho histone h3, by Merck Group (1 mentions) Dm5000b upright microscope, by Leica (1 mentions) Cytoseal xyl, by Thermo Fisher Scientific (1 mentions) Dm2500, by Leica (1 mentions)

Close spelling variants of this product

Application Suite software X Applications Suite software Leica Applications Suite Leica Application Suite Leica application software Software Suite Leica Application

3 protocols using applications suite software

Quantifying Proliferation and Apoptosis

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Proliferation and apoptotic indices were calculated by counting the numbers of phospho-histone-H3 (1:500, 06–570, Millipore), or cleaved caspase 3 (1:500, 9661, Cell signalling)-positive cells, respectively, in 10 comparable fields per mouse kidney or gene knockdown, as a percentage of DAPI-stained nuclei (n = 4 biological samples per genotype or group). Immunofluorescence analysis was carried out using a Leica DM2500 optical microscope with the Leica applications suite software. Adobe Photoshop CS7 software was used to compose and analyse the images.

Richards T., Modarage K., Dean C., McCarthy-Boxer A., Hilton H., Esapa C., Norman J., Wilson P, & Goggolidou P. (2019). Atmin modulates Pkhd1 expression and may mediate Autosomal Recessive Polycystic Kidney Disease (ARPKD) through altered non-canonical Wnt/Planar Cell Polarity (PCP) signalling. Biochimica et Biophysica Acta. Molecular Basis of Disease, 1865(2), 378-390.

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Histological Analysis of Mouse Organs

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Mouse lung, liver and kidney samples collected after the 46 day in vivo study were fixed in 10% formalin for 48 hours then placed in two changes of 70% ethanol for storage. Samples were then embedded in paraffin and cut into sections of between 5–7 μm. Rehydration was performed, followed by staining with hematoxylin, differentiation in acidic alcohol (0.1%), staining with eosin counterstain, dehydration with 100% ethanol, a xylene rinse, and finally mounting with Cytoseal™ XYL (Thermo Scientific, Waltham, MA, USA). Samples were imaged using a Leica DM 5000B upright microscope using the Leica Applications Suite software (Leica, Buffalo Grove, IL, USA).

Brinkman A.M., Chen G., Wang Y., Hedman C.J., Sherer N.M., Havighurst T.C., Gong S, & Xu W. (2016). Aminoflavone-Loaded EGFR-Targeted Unimolecular Micelle Nanoparticles Exhibit Anti-Cancer Effects in Triple Negative Breast Cancer. Biomaterials, 101, 20-31.

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Histopathological Evaluation of Pancreas

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On the basis of results, only the control groups and high dose (200 mg/kg) treated animal groups were shortlisted for the histopathological examinations. Isolated pancreas of sacrificed animals were fixed in 10% formalin solution and immediately processed by the paraffin technique. Sections of 4 μm thickness were cut and stained by hematoxylin and eosin (H and E) for histopathological examination [27] (link). Well stained sections of pancreas were studied with the help of Leica digital microscope (Leica DM 2500, China) with Leica applications suite software. Pancreatic tissue and islets were observed for their necrosis and/or regeneration.

Itankar P.R., Tauqeer M, & Dalal J.S. (2018). Toxicological and pharmacological profiling of organically and non-organically cultivated Cymbopogon citratus. Journal of Ayurveda and Integrative Medicine, 10(4), 233-240.

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