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Bond epitope retrieval 2

Manufactured by Cell Signaling Technology
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Bond Epitope Retrieval 2 is a laboratory equipment product designed for antigen retrieval in immunohistochemical (IHC) and immunofluorescence (IF) applications. It utilizes a thermal-induced epitope retrieval (TIER) method to unmask antigenic sites within formalin-fixed, paraffin-embedded (FFPE) tissue sections.

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Lab products found in correlation

Bond polymer kit, by Cell Signaling Technology (2 mentions) Bond 3 system, by Leica (2 mentions) E1l3n, by Cell Signaling Technology (2 mentions)

2 protocols using bond epitope retrieval 2

1

Automated PD-L1 Immunohistochemistry in FFPE Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
This was performed on FFPE tissue sections using an automated immunostainer (Bond-III system, Leica Biosystems). Following antigen retrieval by combination of heat and Bond Epitope Retrieval 2 solution for 20 minutes, PD-L1 was stained with the monoclonal antibody clone E1L3N (Cell Signalling) and visualized using Bond Polymer Kit.
Wu F., Watanabe N., Tzioni M.M., Akarca A., Zhang C., Li Y., Chen Z., Cucco F., Carmell N., Noh J.Y., Ito K., Dobson R., Moody S., Yao W., Zhang W., Liu W., Liu H., Okosun J., Chott A., Bi Y., Chuang S.S., Raderer M., Li J.Y., Marafioti T, & Du M.Q. (2021). Thyroid MALT lymphoma: self-harm to gain potential T-cell help. Leukemia, 35(12), 3497-3508.
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2

Automated PD-L1 Immunohistochemistry on FFPE

Check if the same lab product or an alternative is used in the 5 most similar protocols
This was performed on FFPE tissue sections using an automated immunostainer (Bond-III system, Leica Biosystems). Following antigen retrieval by combination of heat and Bond Epitope Retrieval 2 solution for 20 min, PD-L1 was stained with the monoclonal antibody clone E1L3N (Cell Signalling) and visualised using Bond Polymer Kit.
Wu F., Watanabe N., Tzioni M.M., Akarca A., Zhang C., Li Y., Chen Z., Cucco F., Carmell N., Noh J.Y., Ito K., Dobson R., Moody S., Yao W., Zhang W., Liu W., Liu H., Okosun J., Chott A., Bi Y., Chuang S.S., Raderer M., Li J.Y., Marafioti T, & Du M.Q. (2021). Thyroid MALT lymphoma: self-harm to gain potential T-cell help. Leukemia, 35(12), 3497-3508.
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