Cardiac and cardiac myocyte injury were quantified by measuring levels of cardiac troponin-I ELISA (Life Diagnostics, West Chester, PA) in plasma and culture supernatant, respectively, as described.[26 (link)] A commercial ELISA was used to measure levels of nitrotyrosine in protein (Hycult, Plymouth Meeting, PA). Acetylation of p53, as marker for apoptosis, was measured with the PathScan® Acetylated p53 Sandwich ELISA Kit (Cell Signaling, Danvers, MA). CXCL1 and myeloperoxidase protein levels in heart lysates were analyzed using a Duo-Set® (R&D Systems).[25 (link)] All assays were performed according to the manufacturers’ instructions.
Cardiac troponin 1 elisa
Manufactured by Life Diagnostics
Sourced in United Kingdom
The Cardiac troponin-I ELISA is a laboratory test used to measure the levels of cardiac troponin-I, a protein that is released into the bloodstream when the heart muscle is damaged. The test is designed to aid in the diagnosis and monitoring of various cardiac conditions, such as myocardial infarction (heart attack).
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Lab products found in correlation
2 protocols using cardiac troponin 1 elisa
1
Cardiac Injury Quantification and Apoptosis Markers
2
Murine Coronary Artery Ligation Model
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CAL was performed in wild-type C57/BL6 mice as described previously [19 ,20 (link)]. In brief, an incision was made in the lower thorax and the chest opened at the fourth intercostal space and held open using retractors. The left anterior descending coronary artery was ligated in mice randomised to the CAL group, at the level below the left atrium. After ligation, the chest was closed. For mice undergoing sham surgery, thoracotomy alone was performed; ligation of the left anterior descending coronary artery was not conducted. An ultra-sensitive mouse cardiac troponin-I ELISA (Life Diagnostics, Stoke on Trent, UK) was used on serum as per the manufacturer's instructions.
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