All the chemicals and reagents were commercially available and used as received without further purification. Superdry tetrahydrofuran (THF) was purchased from J&K Scientific. Titanium tetrachloride (TiCl4), 4-dimethylaminopyridine (DMAP), 4-hydroxybenzophenone, and n-butyllithium were purchased from Aldrich. Dicyclohexylcarbodiimide (DCC), p-toluene sulfonic acid (PTSA) monohydrate, and Boc-l -glutamic acid 1-tert-butyl ester (Boc-Glu-OtBu) were purchased from Acros Organics. Lipases from Pseudomonas cepacia (PCL), Candida rugosa (CRL), Candida Antarctica B (CALB), Pseudomonas fluorescens (PFL), Porcine pancreas (PPL), and Aspergillus niger (ANL) were all ordered from Amano Enzymes. Glucose oxidase, alkaline phosphatase, acylase, laccase, and α-amylase were all purchased from Sigma-Aldrich. The Lipase Assay Kit was purchased from the Nanjing Jiancheng Bioengineering Institute. The human serum samples were supplied by Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, and the serum samples were from both healthy people and acute pancreatitis patients. The water used in the tests was triple distilled, treated with an ion exchange column, and then treated with a Milli-Q water purification system.
Acylase
Manufactured by Merck Group
Sourced in United States
Acylase is a laboratory enzyme used to catalyze the hydrolysis of acyl groups from various substrates. It functions by breaking down the chemical bonds in acyl compounds, facilitating their separation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using acylase
1
Enzymatic Assay Development for Pancreatitis
2
Multispecies Consortium Biofilm Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A consortium composed of Acinetobacter sp. YS01, Bacillus sp. AS03, Escherichia coli, Enterobacter sp. YS02, Sphingobium xanthum, Sphingopyxis sp. NM1, Microbacterium sp. NM2, Staphylococcus warneri, and Xanthomonas translucens was used as an inoculum. The different daily acylase treatment episodes are shown in Table S2 . One hundred microliters of the consortium, with or without acylase (Sigma-Aldrich, USA; final concentration of 10 mg/l), was inoculated onto the bottom of a plate. The plate was incubated at 25°C with agitation at 40 rpm for 5 days, during which the suspension was replaced daily with the same volume of fresh LS-R2A medium with or without acylase (final concentration, 10 mg/l). There were three replicates per each treatment.
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