Analytical standards of solanine,
chaconine, solasonine, solamargine, tomatine and their aglycons solanidine,
solasodine, and tomatidine were purchased from Sigma-Aldrich (Steinheim,
Germany), Carl Roth (Karlsruhe, Germany), and Extrasynthese (Genay,
France). The standards were solved in methanol, obtaining stock solutions
of 1 mM for storage at −18 °C, respectively.
The
solvents used for high-performance liquid chromatography coupled to
quadrupole time-of-flight mass spectrometery (HPLC–QToF–MS)
and high-performance liquid chromatography coupled to tandem mass
spectrometery (HPLC–MS/MS) analysis (HPLC–MS grade)
and all other chemicals used for incubation experiments were purchased
from Fisher Scientific (Schwerte, Germany), Merck (Darmstadt, Germany),
or Sigma-Aldrich (Steinheim, Germany) in gradient or reagent quality.
Purified water was obtained by a Purelab flex 2 system (ELGA LabWater
Veolia Water Technologies, Celle, Germany).
chaconine, solasonine, solamargine, tomatine and their aglycons solanidine,
solasodine, and tomatidine were purchased from Sigma-Aldrich (Steinheim,
Germany), Carl Roth (Karlsruhe, Germany), and Extrasynthese (Genay,
France). The standards were solved in methanol, obtaining stock solutions
of 1 mM for storage at −18 °C, respectively.
The
solvents used for high-performance liquid chromatography coupled to
quadrupole time-of-flight mass spectrometery (HPLC–QToF–MS)
and high-performance liquid chromatography coupled to tandem mass
spectrometery (HPLC–MS/MS) analysis (HPLC–MS grade)
and all other chemicals used for incubation experiments were purchased
from Fisher Scientific (Schwerte, Germany), Merck (Darmstadt, Germany),
or Sigma-Aldrich (Steinheim, Germany) in gradient or reagent quality.
Purified water was obtained by a Purelab flex 2 system (ELGA LabWater
Veolia Water Technologies, Celle, Germany).