Hek293t cell line

SH-SY5Y human neuroblastoma cells were grown in 1:1 mixture of MEM and F12, supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1 mM sodium pyruvate, 0.1 mM nonessential amino acid, 1.5 g/L sodium bicarbonate, 100 units/mL penicillin, and 100 μg/mL streptomycin. All media and supplements were purchased from Gibco (Gaithersburg, MD, USA). Cells were maintained at 37 °C in a humidified atmosphere of 5% CO2. Cells were treated with D-mannose for the 12 h before RNA or 24 h before protein extraction.
HEK293T cell line was purchased from Shanghai Cell Bank of Chinese Academy of Sciences (GNHu17) and grown in DMEM medium (Gibco, 12100046) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, 10099–141). Cells were all cultured in a humidified cell incubator with an atmosphere of 5% CO2 at 37 °C.
Hela cell line was purchased from ATCC (American Type Culture Collection) and grown in DMEM medium (Gibco, 12100046) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, 10099–141). Cells were all cultured in a humidified cell incubator with an atmosphere of 5% CO2 at 37 °C.

SGC-7901 and MGC-803 human gastric cancer cell line, HEK-293T cell line, and human skin fibroblast HSF cell line were purchased from the Cell Bank of Shanghai (Shanghai, China) and cultured in Dulbecco minimum essential medium (DMEM), supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Hyclone, Logan, UT, USA), at 37 °C in a humidified atmosphere with 5% CO₂.