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αpol 2

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αPol II is a monoclonal antibody that recognizes the C-terminal domain of the RNA polymerase II (Pol II) enzyme. This antibody is commonly used in various applications, such as immunoprecipitation and Western blotting, to detect and study the Pol II enzyme, which is responsible for the transcription of protein-coding genes in eukaryotic cells.

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Lab products found in correlation

α flag, by Merck Group (2 mentions) α gapdh, by Santa Cruz Biotechnology (2 mentions) 17β estradiol e2, by Merck Group (2 mentions) Anti ha magnetic beads, by Thermo Fisher Scientific (2 mentions) Dynabeads protein a, by Thermo Fisher Scientific (2 mentions) Itaq universal sybr green supermix, by Bio-Rad (2 mentions) Streptavidin beads, by New England Biolabs (2 mentions) Fulvestrant from, by MedChemExpress (2 mentions) Rnase a t, by Thermo Fisher Scientific (2 mentions) αkdm2a, by Proteintech (2 mentions) αrad21, by Abcam (2 mentions) αlamina c, by Abcam (2 mentions) Hispur cobalt superflow agarose beads, by Thermo Fisher Scientific (2 mentions) Rnase t1 cocktail, by Thermo Fisher Scientific (2 mentions)

2 protocols using αpol 2

1

Antibodies and Reagents for Epigenetic Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study include: αERα (Bethyl #A300–495A) for ChIP-qPCR, ChIP-Seq, RIP-qPCR, PAR-CLIP and immunoblotting; αKDM2A (ProteinTech #24311–1-AP) for ChIP-qPCR; αPol II (Santa Cruz Biotechnology #SC-9001, #SC-56767 and Abcam #ab817) for ChIP-qPCR; αRAD21 (Abcam #ab992) for ChIP-qPCR. Other antibodies that were applied in western blot include: αGST (ProteinTech #66001–2); αHis (ProteinTech #66005–1); αHA (BioLegend, #901501); αNEDD4 (CST #3607); αPol II (Santa Cruz Biotechnology #SC-56767), αLaminA/C (Abcam, # ab8984), αGAPDH (Santa Cruz Biotechnology #sc-365062) and αFLAG (Sigma #F1804). Reagents that were utilized for immunoprecipitation purposes are: anti-HA magnetic beads (Thermo Fisher #88837); streptavidin beads (NEB #S1420S); HisPurTM cobalt Superflow agarose beads (Thermo Fisher #25228); Dynabeads Protein A (Thermo Fisher #10006D) or Protein G (Thermo Fisher #10007D). Normal anti-rabbit IgG antibody was purchased from Invitrogen (#02–6102), 17β-estradiol (E2) from Sigma (#E2758), fulvestrant from Medchem Express (#HY13636), RNase A/T from Thermo Fisher (#EN0551), RNase/T1 cocktail from Thermo Fisher (#AM2286) and iTaq Universal SYBR Green Supermix from BioRad (1725124). Antibodies information can also be found in Key Resources Table.
Yang M., Lee J.H., Zhang Z., De La Rosa R., Bi M., Tan Y., Liao Y., Hong J., Du B., Wu Y., Scheirer J., Hong T., Li W., Fei T., Hsieh C.L., Liu Z., Li W., Rosenfeld M.G, & Xu K. (2020). Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor. Cell reports, 31(12), 107803.
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2

Antibodies and Reagents for Epigenetic Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study include: αERα (Bethyl #A300–495A) for ChIP-qPCR, ChIP-Seq, RIP-qPCR, PAR-CLIP and immunoblotting; αKDM2A (ProteinTech #24311–1-AP) for ChIP-qPCR; αPol II (Santa Cruz Biotechnology #SC-9001, #SC-56767 and Abcam #ab817) for ChIP-qPCR; αRAD21 (Abcam #ab992) for ChIP-qPCR. Other antibodies that were applied in western blot include: αGST (ProteinTech #66001–2); αHis (ProteinTech #66005–1); αHA (BioLegend, #901501); αNEDD4 (CST #3607); αPol II (Santa Cruz Biotechnology #SC-56767), αLaminA/C (Abcam, # ab8984), αGAPDH (Santa Cruz Biotechnology #sc-365062) and αFLAG (Sigma #F1804). Reagents that were utilized for immunoprecipitation purposes are: anti-HA magnetic beads (Thermo Fisher #88837); streptavidin beads (NEB #S1420S); HisPurTM cobalt Superflow agarose beads (Thermo Fisher #25228); Dynabeads Protein A (Thermo Fisher #10006D) or Protein G (Thermo Fisher #10007D). Normal anti-rabbit IgG antibody was purchased from Invitrogen (#02–6102), 17β-estradiol (E2) from Sigma (#E2758), fulvestrant from Medchem Express (#HY13636), RNase A/T from Thermo Fisher (#EN0551), RNase/T1 cocktail from Thermo Fisher (#AM2286) and iTaq Universal SYBR Green Supermix from BioRad (1725124). Antibodies information can also be found in Key Resources Table.
Yang M., Lee J.H., Zhang Z., De La Rosa R., Bi M., Tan Y., Liao Y., Hong J., Du B., Wu Y., Scheirer J., Hong T., Li W., Fei T., Hsieh C.L., Liu Z., Li W., Rosenfeld M.G, & Xu K. (2020). Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor. Cell reports, 31(12), 107803.
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