GHS was incubated in a fluorescein isothiocyanate–dextran solution (Sigma, MO, USA, with an average molecular weight of 2 MDa) in Ultra‐Pure Milli‐Q water (15 µM). The scaffolds were then imaged using a Leica DMi8 Thunder microscope (Germany). The Leica application suite X (LAS X, version 3.7.4.23463) software was used to construct 3D images from high‐magnification (250×) z‐stacked images (141 z‐slices, increment size = 0.502 µm). The void fraction was then measured as the fraction of occupied void space over the total volume, using LAS X software. Also, for pore detection and reporting equivalent median pore diameter, a MATLAB code was developed and used, wherein 2D fluorescence microscopy images were thresholded, and the pores were detected and masked. The areas of detected pores were obtained and converted to equivalent‐area circles, from which the diameters were calculated. The median of the equivalent diameter distribution was reported.
Fluorescein isothiocyanate dextran solution
Manufactured by Merck Group
Sourced in United States
Fluorescein isothiocyanate–dextran solution is a laboratory reagent composed of fluorescein isothiocyanate (FITC) conjugated to dextran. It is used as a fluorescent label for various applications in biological research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fluorescein isothiocyanate dextran solution
1
Quantitative 3D Microscopy of Hydrogels
2
Quantifying Porous Scaffold Microstructure
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GHS were incubated in a fluorescein isothiocyanate–dextran solution (Sigma, MO, USA, with an average molecular weight of 2 MDa) in Ultra-Pure Milli-Q® water (15 μM). The scaffolds were then imaged using a Leica DMi8 Thunder™ microscope (Germany). The Leica application suite X (LAS X, version 3.7.4.23463) software was used to construct 3D images from high-magnification (250×) z-stacked images (141 z-slices, increment size = 0.502 μm). The void fraction was then measured as the fraction of occupied void space over the total volume, using LAS X software. Also, for pore detection and reporting equivalent median pore diameter, a MATLAB code was developed and used, wherein 2D fluorescence microscopy images were thresholded, and the pores were detected and masked. The areas of detected pores were obtained and converted to equivalent-area circles, from which the diameters were calculated. The median of the equivalent diameter distribution was reported.
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