Goat anti mouse peroxidase conjugated antibodies

Western blot of neuronal protein extracts was performed as described previously [48 (link)]. The following primary antibodies were used: rabbit anti-Fbp (1:500, isolated and purified as described in [49 (link)]), rabbit anti-Camk2α (1:1000, Merck KGaA, Darmstadt, Germany, C6974) and mouse anti-β-actin (1:50,000, Merck KGaA, Darmstadt, Germany, A1978). The following secondary antibodies were used: goat anti-rabbit peroxidase-conjugated (1:50,000, Merck KGaA, Darmstadt, Germany, a0545) and goat anti-mouse peroxidase-conjugated antibodies (1:100,000, Merck KGaA, Darmstadt, Germany, a9044) were used. The reaction was visualized chemiluminescently.

Western blot of neuronal protein extracts was performed as described in [20 (link)] using the following antibodies: rabbit anti-Fbp (1:2000, [21 (link)]), mouse anti-Camk2α (1:1000, ThermoFisher Scientific, Waltham, MA, USA, MA1-048) and rabbit anti-tubulin β3 (1:1000, Synaptic System GmbH, Göttingen, Germany, 302302). Goat anti-rabbit peroxidase-conjugated (1:50,000, Merck KGaA, Darmstadt, Germany, a0545) and goat anti-mouse peroxidase-conjugated antibodies (1:50,000, Merck KGaA, Darmstadt, Germany, a9044) were used as secondary antibodies, and the reaction was visualized using the SuperSignal^TM West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific, Waltham, MA, USA).