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Anti 4 hne ab48506

Manufactured by Abcam
Sourced in United States

Anti-4-HNE (ab48506) is a primary antibody that detects 4-Hydroxynonenal (4-HNE), a lipid peroxidation product. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and ELISA, to detect and quantify 4-HNE levels in biological samples.

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2 protocols using anti 4 hne ab48506

1

Ferroptosis Pathway Protein Analysis

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Anti-GSS (sc-166882, RRID: AB_10611504)
antibody was obtained from Santa Cruz, anti-GSS (15712-1-AP, RRID:
AB_2878171); anti-GAPDH (10494-1-AP, RRID: AB_2263076), anti-PTGS2
(66351-1-Ig, RRID: AB_2881731) antibody were obtained from Proteintech;
and anti-GPX4 (ab125066, RRID: AB_10973901), anti-4-HNE (ab48506,
RRID: AB_867452) were obtained from Abcam. Erastin (T1765, 571203-78-6),
RSL3 (T3646, 1219810-16-8) were obtained from TagerMol.
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2

Oxypaeoniflorin Modulates Cellular Pathways

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Oxypaeoniflorin (Oxy) (purity > 98.06%) was purchased from MedChemExpress (#HY-N0748, Shanghai, China). Fetal bovine serum (FBS), trypsin-EDTA (0.25%) phenol red, and Dulbecco's modified Eagle's medium, Nutrient Mixture F-12 (DMEM/F-12), were purchased from Invitrogen-Gibco (Grand Island, NY). Anti-GAPDH (ab8245), anti-Sirt1 (ab189494), anti-Bcl-2 (ab32124), anti-Bax (ab32503), and anti-4-HNE (ab48506) antibodies were obtained from Abcam (Cambridge, MA, USA). The secondary antibody was obtained from LI-COR Biosciences (Lincoln, United States). The QuantiPro ™ Bicinchoninic Acid Protein Assay Kit from Sigma-Aldrich LLC (St. Louis, MO, USA) was employed to analyze protein concentrations. Lipopolysaccharide (LPS) was obtained from Sigma-Aldrich (#L6386-25MG, Beijing Merck Limited, China). All chemical reagents used were of analytical grade in the present study.
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