The following antibodies were used in flow cytometry: PE anti-mouse H-2Kb/H-2Db, PE anti-mouse IFNγR β chain (Biolegend, San Diego, CA, USA), PE anti-mouse CD119 (IFNγ Receptor 1) (eBioscience, San Diego, CA, USA). Antibodies used in western blotting analysis were: iNOS, pTyr701-Stat1, total Stat1, total Stat2, pSer536-p65, total p65 pSer32-IκBα, total IκBα, β-actin, GAPDH (Cell Signaling Technology, Danvers, MA, USA); pTyr690-Stat2 (Abcam, Cambridge, MA, USA); LaminB (Epitomics, Burlingame, CA, USA). Recombinant mouse IFNα, IFNβ, IFNγ and TNFα were from R&D Systems (Minneapolis, MN, USA). L-NMMA was from Sigma-Aldrich (St. Louis, MO, USA).
Total p65 pser32 iκbα
Manufactured by Cell Signaling Technology
Sourced in United States
Total p65 pSer32-IκBα is an ELISA kit that quantifies the levels of phosphorylated IκBα at Serine 32 in cellular lysates. IκBα is a key regulator of the NF-κB signaling pathway, and its phosphorylation at Ser32 is an important event in the activation of NF-κB.
Automatically generated - may contain errors
Lab products found in correlation
L nmma, by Merck Group (2 mentions)
Total stat1, by Cell Signaling Technology (2 mentions)
Pe anti mouse h 2kb h 2db, by BioLegend (2 mentions)
Ptyr701 stat1, by Cell Signaling Technology (2 mentions)
Total stat2, by Cell Signaling Technology (2 mentions)
Total iκbα, by Cell Signaling Technology (2 mentions)
Pe anti mouse ifnγr β chain, by BioLegend (2 mentions)
Ptyr690 stat2, by Abcam (2 mentions)
Lamin b, by Abcam (2 mentions)
Recombinant mouse ifnα, by R&D Systems (2 mentions)
P p65 ser536, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Tnf α, by R&D Systems (2 mentions)
Ifn γ, by R&D Systems (2 mentions)
Ifn β, by R&D Systems (2 mentions)
2 protocols using total p65 pser32 iκbα
1
Flow Cytometry and Western Blot Analysis of Interferon Signaling
2
Flow Cytometry and Western Blotting of IFN Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies were used in flow cytometry: PE anti-mouse H-2Kb/H-2Db, PE anti-mouse IFNγR β chain (Biolegend, San Diego, CA, USA) and PE anti-mouse CD119 (IFNγ Receptor 1) (eBioscience, San Diego, CA, USA). Antibodies used in western blotting analysis were iNOS, pTyr701-Stat1, total Stat1, total Stat2, pSer536-p65, total p65 pSer32-IκBα, total IκBα, β-actin, GAPDH (Cell Signaling Technology, Danvers, MA, USA); pTyr690-Stat2 (Abcam, Cambridge, MA, USA) and LaminB (Epitomics, Burlingame, CA, USA). Recombinant mouse IFNα, IFNβ, IFNγ and TNFα were from R&D Systems (Minneapolis, MN, USA). l -NMMA was from Sigma-Aldrich (St Louis, MO, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!