PBMC were isolated from non-cancer controls by density gradient centrifugation and seeded at a density of 1 × 106 cells/mL cRPMI. Cells were cultured with OAC patient- derived ACM for 2 and 24 h or M199. Cells were stained with CD56-FITC-Viobright (Miltenyi Biotec), L-selectin-BV510, CD3-APC-Cy7, Integrin β7-Pe-Cy5, α4-Pe-Cy7 (Biolegend). Samples were acquired using the CANTO II (BD Biosciences) flow cytometer and analysed using FlowJo software (BD Biosciences).
L selectin bv510
Manufactured by BioLegend
Sourced in Germany
L-selectin-BV510 is a fluorescent-conjugated antibody that binds to the L-selectin receptor. L-selectin is a cell adhesion molecule involved in the initial tethering and rolling of leukocytes on endothelial cells, a crucial step in the inflammatory response. The BV510 fluorescent dye allows for detection and analysis of L-selectin-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 apc cy7, by BioLegend (3 mentions)
Cd56 fitc viobright, by Miltenyi Biotec (3 mentions)
Integrin β7 pe cy5, by BioLegend (3 mentions)
α4 pe cy7, by BioLegend (3 mentions)
Canto 2, by BD (3 mentions)
Trail apc, by BioLegend (2 mentions)
Fasl bv421, by BioLegend (2 mentions)
Recombinant fractalkine, by BioLegend (1 mentions)
Lysing solution, by BD (1 mentions)
3 protocols using l selectin bv510
1
Profiling Immune Cells in Ovarian Cancer
2
Fractalkine and IL-15 Stimulation of PBMCs
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PBMC were isolated from non-cancer controls by density gradient centrifugation and seeded at a density of 1 × 106 cells/mL cRPMI. Cells were treated with 30 ng/mL of recombinant fractalkine (Biolegend) and/or 100 ng/mL of recombinant IL-15 (Immunotools, Friesoythe, Germany) for 2 or 24 h. Cells were stained with CD56-FITC-Viobright (Miltenyi Biotec), L-selectin-BV510, CD3-APC-Cy7, Integrin β7-Pe-Cy5, α4-Pe-Cy7, TRAIL-APC, FasL-BV421 (Biolegend). Samples were acquired using the CANTO II (BD Biosciences) flow cytometer and analysed using FlowJo software (BD Biosciences).
3
Comprehensive Immune Cell Analysis
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Whole blood, SVF from omentum and intratumoural immune cells were stained with CD56-FITC-Viobright (Miltenyi Biotec, Bergisch Gladbach, Germany), L-selectin-BV510, CD3-APC-Cy7, Integrin β7-Pe-Cy5, α4-Pe-Cy7, TRAIL-APC, FasL-BV421 (Biolegend, San Diego, CA, USA). Red blood cells were lysed using BD Lysing Solution (BD Biosciences, Franklin Lakes, NJ, USA) as per manufacturer’s instructions. NK cells were quantified as CD56+CD3- cells within the lymphocyte gate. Cells were acquired using the CANTO II (BD Biosciences) flow cytometer and analysed using FlowJo software (BD Biosciences).
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