To determine the neutralization mechanism of MAb 22.9-1, binding competition experiments were conducted by SPR assay with the Biacore 8K instrument and NTA chip (Cytiva). Purified hACE2 (Sino Biological) was immobilized at a concentration of 10 μg/mL. The following samples were injected: (i) 40 μg/mL O-RBD, (ii) complex of 40 μg/mL O-RBD and 0.15 mg/mL MAb 22.9-1, (iii) complex of 40 μg/mL O-RBD and 0.15 mg/mL MAb 20.8-8. The data were analyzed with the Biacore Insight Evaluation software. The curves were plotted using GraphPad Prism software.
Nta chip
Manufactured by Cytiva
The NTA chip is a laboratory equipment used for the analysis and characterization of biomolecular interactions. It is designed to measure binding affinities and kinetics between proteins and other analytes. The chip surface is coated with nitrilotriacetic acid (NTA), which allows for the immobilization of proteins or other molecules of interest through metal-affinity interactions.
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Lab products found in correlation
2 protocols using nta chip
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Neutralization Mechanism of SARS-CoV-2 Monoclonal Antibody
2
Neutralization Mechanism of SARS-CoV-2 Monoclonal Antibody
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To determine the neutralization mechanism of MAb 22.9-1, binding competition experiments were conducted by SPR assay with the Biacore 8K instrument and NTA chip (Cytiva). Purified hACE2 (Sino Biological) was immobilized at a concentration of 10 μg/mL. The following samples were injected: (i) 40 μg/mL O-RBD, (ii) complex of 40 μg/mL O-RBD and 0.15 mg/mL MAb 22.9-1, (iii) complex of 40 μg/mL O-RBD and 0.15 mg/mL MAb 20.8-8. The data were analyzed with the Biacore Insight Evaluation software. The curves were plotted using GraphPad Prism software.
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