The antibodies used included the polyclonal rabbit antisera against Brg1 that was previously described (40 (link)). The rabbit anti-casein kinase IIα (H-286), mouse anti-casein kinase IIα′ (D-7), mouse anti-casein kinase IIβ (6D5), goat anti-Pax-3/7 antibody (N-19), rabbit anti-caspase-3 antibody (H-277), rabbit anti-Baf155 antibody (H-76), rabbit anti-Baf170 antibody (H-116), rabbit anti-lamin β1 (H-90), rabbit anti-pol II (N-20), and rabbit anti-MyoD (C-20) were purchased from Santa Cruz Biotechnology, Inc. Rabbit anti-PI3K p85 antibody, N-SH2 domain (ABS233) was obtained from Millipore Corp. Mouse anti-GAPDH-peroxidase-conjugated (G9295) was obtained from Sigma. Anti-myosin heavy chain (MF20, deposited by D. A. Fischman) antibody was obtained as a hybridoma supernatant from the Developmental Studies Hybridoma Bank (University of Iowa). Donkey anti-rabbit Alexa-488 (A21206) fluorescent secondary antibody was purchased from Thermo Fisher Scientific. Secondary antibodies anti-mouse and anti-rabbit HRP-coupled secondary antibodies were obtained from Pierce.
Rabbit anti myod c 20
Manufactured by Santa Cruz Biotechnology
Rabbit anti-MyoD (C-20) is an antibody product produced by Santa Cruz Biotechnology. It is designed to detect MyoD, a transcription factor that plays a critical role in the regulation of muscle cell differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti pol 2 n 20, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh peroxidase conjugated g9295, by Merck Group (1 mentions)
Horse serum, by Cedarlane (1 mentions)
Infinity 3 camera, by Teledyne (1 mentions)
Alexa flour 647, by Jackson ImmunoResearch (1 mentions)
Anti ki67, by Abcam (1 mentions)
Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions)
Alexa 488, by Jackson ImmunoResearch (1 mentions)
2 protocols using rabbit anti myod c 20
1
Antibody Characterization and Validation
2
Immunofluorescence Analysis of Myofibers and Myoblasts
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Myofibers were fixed in 4% PFA in PBS, 1% glycine, and blocked in PBS containing 0.2% Triton X-100 (BioShop), 2% BSA, 5% horse serum (Cedarlane), 1% azide. Myoblasts were fixed in 2% PFA in PBS and blocked in PBS containing 0.3% Triton X-100 and 10% goat serum. The primary antibodies used for detection were mouse anti-PAX7 (DSHB, 1/100), rabbit anti-MYOD (c-20) (Santa Cruz, catalog no. sc-304), MYH (Santa Cruz, catalog no. sc-20641), or anti-Ki67 (Abcam). Cells were washed with PBS and incubated in mouse anti-biotin and streptavidin-Cy3 or secondary antibodies conjugated to a fluorescent dye (Cy3, Alexa 488 or Alexa Flour 647; all from Jackson ImmunoResearch). Nuclei were counterstained with DAPI (0.5 μg/mL). Pictures were acquired using a Leica DM 3000B microscope and Infinity-3 camera (Lumenera).
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