Prism 5.0 statistical

Manufactured by GraphPad

Prism 5.0 is a statistical software application developed by GraphPad. It is designed to analyze and visualize data, with a focus on statistical analysis and graphing capabilities.

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Lab products found in correlation

Qiaamp dna blood and tissue mini kit, by Qiagen (1 mentions) Pentamidine, by Merck Group (1 mentions) Amphotericin b, by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)

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GraphPad 5.0 Prism Prism statistical GraphPad Prism 5

4 protocols using prism 5.0 statistical

Student Population Sampling Study

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The sample was selected according to data obtained from the Municipal Secretariat of Education of the city of Lagarto, who reported that the municipality had about 4590 students attending the final years of elementary school at the beginning of the 2018 school year, Barbetta [21 ]

n = \frac{N \times n 0}{N + n 0},

considering a sampling error of 5% of the target population, totaling a sample of 230 students (13 to 16 years old), distributed between the two larger schools in the urban area, to be evaluated during the survey. However, at the end of the research, only one school was studied, due to structural and strategic impediments. We obtained 122 participants from which the research data were evaluated, which represents, on average, 3% of the total population (Figure 1).
The data obtained from the application of the anamnesis questionnaires and registrations and the results will be expressed as means, standard deviations and confidence intervals. A previous significance level of 5% was established with a value of p ≤ 0.05; the results were graphically illustrated using Prism 5.0 statistical software. To ensure the privacy and integrity of students, the applied questionnaires were given codes for the analysis and reporting of results from laboratory tests.

da Rosa Santos L., de Araujo S.S., Vieira E.F., Estevam C.D., dos Santos J.L., Wichi R.B., Lima F.B., Carvalho C.R., Aidar F.J, & Marçal A.C. (2020). Effects of 12 Weeks of Resistance Training on Cardiovascular Risk Factors in School Adolescents. Medicina, 56(5), 220.

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Comparative Analysis of Cellular Response

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The data were presented as mean standard deviation (M±SD). The statistical signi cance was assessed by one-way analysis of variance (ANOVA), followed by multiple comparisons using Dunnet's test to determine the statistical signi cance between groups. When p < 0.05, the difference was considered statistically signi cant. Prism 5.0 statistical software was used to analyze all data.

Chen H.L., Yang L., Zhang X.L., Jia Q.Y., Duan Z.D., Li J.J., Zheng L.Y., Liu T.T., Qi Z., Yuan Y, & Wu C.Y. (2023). Scutellarin Acts via MAPKs Pathway to Promote M2 Polarization of Microglial Cells. Molecular neurobiology, 60(8).

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Quantification of HSV DNA in NHP Samples

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NHP samples for qPCR analysis (25 mg) were placed in sterile microcentrifuge tubes in proteinase K containing digestion buffer and digested overnight at 56°C. DNA was extracted into 200 μL elution buffer from NHP samples using the QIAamp DNA Blood and Tissue Mini Kit (QIAGEN) per the manufacturer’s instructions. The tissue preparation, DNA extraction, and TaqMan preparation steps were performed in a laminar flow hood in a laboratory that does not work with HSVs. Extracted DNA samples were incubated with the following HSV-specific primers and probes for HSV polymerase (sequences kindly provided by Dr. Fred Lakeman): PolF (forward), 5′-ACC GCC GAA CTG AGC AGA C-3′; and PolR (reverse), 5′-TGA GCT TGT AAT ACA CCG TCA GGT-3′. The fluorescent-labeled probe sequence was 5′-6FAM-CGC GTA CAC CAA CAA GCG CCT G-TAMRA-3′. HSV GEs of the amplified product were measured from triplicate samples using an ABI 7300 TaqMan machine (Applied Biosystems ) and compared against logarithmic dilutions of a positive control DNA sequence (10⁶ – 10¹ copies). Descriptive statistical analyses (mean and SD) were used to compare differences in DNA copy numbers between samples using Prism 5.0 statistical software (GraphPad).

Cassady K.A., Bauer D.F., Roth J., Chambers M.R., Shoeb T., Coleman J., Prichard M., Gillespie G.Y, & Markert J.M. (2017). Pre-clinical Assessment of C134, a Chimeric Oncolytic Herpes Simplex Virus, in Mice and Non-human Primates. Molecular Therapy Oncolytics, 5, 1-10.

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Evaluating Leishmania Antiparasitic Efficacy

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Promastigote forms of L. (L.) amazonensis in the logarithmic growth phase (10⁶ parasites/mL) were added to 96-well plates and incubated with SAU 1 and the lanthanide complexes 2–7 (0.78–50.0 µg·mL⁻¹) in six replicates. The microplates were incubated at 26 °C for 72 h. Cell viability was assessed by adding 1 mg·mL⁻¹/well of MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma-Aldrich^® São Paulo, Brazil) [47 (link)]. Results were expressed as the half-maximal inhibitory concentration (IC₅₀) calculated from a non-linear dose–response regression curve using the GraphPad PRISM 5.0 statistical program. Pentamidine (Sigma-Aldrich ^® São Paulo, Brazil; 0.19–12.5 µg·mL⁻¹) and AMB (Amphotericin B, Sigma-Aldrich ^® São Paulo, Brazil; 0.15–1 µg·mL⁻¹) were used as positive controls and pure Schneider^® culture medium as negative control.

da Silva F., Rizk Y.S., das Neves A.R., Lourenço E.M., Ferreira A.M., Monteiro M.M., de Lima D.P., Perdomo R.T., Bonfá I.S., Toffoli-Kadri M.C., Duarte A.P., Nunes D.M., Martines M.A., Piranda E.M, & de Arruda C.C. (2023). Antileishmanial Activity, Toxicity and Mechanism of Action of Complexes of Sodium Usnate with Lanthanide Ions: Eu(III), Sm(III), Gd(III), Nd(III), La(III) and Tb(III). International Journal of Molecular Sciences, 25(1), 413.

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