Plasma was generated from patient blood samples by centrifugation (2500× g for 15 min) and was frozen at −80 °C immediately. Enzyme-linked immunosorbent assays (ELISAs) were performed according to respective instruction manuals to determine mediators of hemostasis and inflammation. Tissue plasminogen activator (t-PA) Combi Actibind, as well as plasminogen activator inhibitor-1 (PAI-1) and t-PA–PAI-1 complex, ELISA kits were purchased from Technoclone (Vienna, Austria). An ELISA kit from USCN (USCN Life Sciences Inc., Wuhan, China) was used to determine plasmin–antiplasmin (PAP) complex levels in plasma. An activated protein C–protein C inhibitor (APC–PCI) complex kit was obtained from BioPorto diagnostics (Hellerup, Denmark) and soluble thrombomodulin was measured with a kit from Asserachrom (Diagnostica Stago, Asnieres, France). Interleukin-6 levels were measured with an Immulite 1000 device (Siemens Healthcare, Erlangen, Germany). Thrombin–antithrombin (TAT) complex was determined with the Enzygnost© TAT Micro-kit from Siemens (Siemens Healthcare, Erlangen, Germany). Furthermore, ELISA measurements of histonylated, citrullinated DNA fragments (Cell Death Detection ELISA kit-plus, Roche, Basel, Switzerland) and PMN Elastase (alpha 1PI-complex, Milenia Biotec, Nauheim, Germany) were performed.
Immulite 1000 device
Manufactured by Siemens
Sourced in Germany, United States
The Immulite 1000 is an automated immunoassay analyzer designed for clinical diagnostic testing. It is capable of performing a variety of immunoassay tests, including those for thyroid function, infectious diseases, and hormones. The device utilizes a chemiluminescent detection method to measure the concentration of analytes in patient samples.
Automatically generated - may contain errors
2 protocols using immulite 1000 device
1
Plasma Mediators of Hemostasis and Inflammation
2
Biomarker Profiling in Exercise Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine ALB, TP, NAG, β-2M, and Cr were assayed with the following techniques:
-albumin with the immunoturbidimetric technique, using ROCHE reagents and Cobas Integra 400/800 Analyzer; -TP was assayed with pyrogallol red, using ROCHE reagents and Integra 400/800; -urine NAG was assayed with the colometric technique [15] (link), using Sigma Aldrich (USA) reagents, measuring concentration of the released p-nitrophenyl-2acetamide-2-desoxy-β-2-glucopiranoside; results were read with Perkin Elmer, USA spectrometer; -β-2M was assayed with the chemiluminescence technique using IMMULITE 1000 device (SIEMENS); -Cr was assayed with the Jaffee technique, using ROCHE reagents and Integra 400/800. Training intensity was monitored in both groups using Polar Team-2 Pro, which recorded the heart rate (HR). The procedure consisted of a belt with HR recorder fitted at the chest level, recording HR during the whole training. Then, the HR recorder was detached and put into a device reading changes in HR.
-albumin with the immunoturbidimetric technique, using ROCHE reagents and Cobas Integra 400/800 Analyzer; -TP was assayed with pyrogallol red, using ROCHE reagents and Integra 400/800; -urine NAG was assayed with the colometric technique [15] (link), using Sigma Aldrich (USA) reagents, measuring concentration of the released p-nitrophenyl-2acetamide-2-desoxy-β-2-glucopiranoside; results were read with Perkin Elmer, USA spectrometer; -β-2M was assayed with the chemiluminescence technique using IMMULITE 1000 device (SIEMENS); -Cr was assayed with the Jaffee technique, using ROCHE reagents and Integra 400/800. Training intensity was monitored in both groups using Polar Team-2 Pro, which recorded the heart rate (HR). The procedure consisted of a belt with HR recorder fitted at the chest level, recording HR during the whole training. Then, the HR recorder was detached and put into a device reading changes in HR.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!