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Immulite 1000 device

Manufactured by Siemens
Sourced in Germany, United States

The Immulite 1000 is an automated immunoassay analyzer designed for clinical diagnostic testing. It is capable of performing a variety of immunoassay tests, including those for thyroid function, infectious diseases, and hormones. The device utilizes a chemiluminescent detection method to measure the concentration of analytes in patient samples.

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2 protocols using immulite 1000 device

1

Plasma Mediators of Hemostasis and Inflammation

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Plasma was generated from patient blood samples by centrifugation (2500× g for 15 min) and was frozen at −80 °C immediately. Enzyme-linked immunosorbent assays (ELISAs) were performed according to respective instruction manuals to determine mediators of hemostasis and inflammation. Tissue plasminogen activator (t-PA) Combi Actibind, as well as plasminogen activator inhibitor-1 (PAI-1) and t-PA–PAI-1 complex, ELISA kits were purchased from Technoclone (Vienna, Austria). An ELISA kit from USCN (USCN Life Sciences Inc., Wuhan, China) was used to determine plasmin–antiplasmin (PAP) complex levels in plasma. An activated protein C–protein C inhibitor (APC–PCI) complex kit was obtained from BioPorto diagnostics (Hellerup, Denmark) and soluble thrombomodulin was measured with a kit from Asserachrom (Diagnostica Stago, Asnieres, France). Interleukin-6 levels were measured with an Immulite 1000 device (Siemens Healthcare, Erlangen, Germany). Thrombin–antithrombin (TAT) complex was determined with the Enzygnost© TAT Micro-kit from Siemens (Siemens Healthcare, Erlangen, Germany). Furthermore, ELISA measurements of histonylated, citrullinated DNA fragments (Cell Death Detection ELISA kit-plus, Roche, Basel, Switzerland) and PMN Elastase (alpha 1PI-complex, Milenia Biotec, Nauheim, Germany) were performed.
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2

Biomarker Profiling in Exercise Study

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Urine ALB, TP, NAG, β-2M, and Cr were assayed with the following techniques:
-albumin with the immunoturbidimetric technique, using ROCHE reagents and Cobas Integra 400/800 Analyzer; -TP was assayed with pyrogallol red, using ROCHE reagents and Integra 400/800; -urine NAG was assayed with the colometric technique [15] (link), using Sigma Aldrich (USA) reagents, measuring concentration of the released p-nitrophenyl-2acetamide-2-desoxy-β-2-glucopiranoside; results were read with Perkin Elmer, USA spectrometer; -β-2M was assayed with the chemiluminescence technique using IMMULITE 1000 device (SIEMENS); -Cr was assayed with the Jaffee technique, using ROCHE reagents and Integra 400/800. Training intensity was monitored in both groups using Polar Team-2 Pro, which recorded the heart rate (HR). The procedure consisted of a belt with HR recorder fitted at the chest level, recording HR during the whole training. Then, the HR recorder was detached and put into a device reading changes in HR.
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