Sep pak light accell plus qma cartridge

The radiosyntheses were conducted on an AllInOne PET tracer radiosynthesiser (Trasis, Liege, Belgium) with 36 manifold actuators and an integrated HPLC (Trasis) equipped with a Chromolith Performance RP18_e column (100 × 10 mm). The general setup of the cassette is depicted in Figure 6 showing connections of tubings, syringes and other disposables. The cassettes were assembled in-house from standard disposable materials supplied by Trasis. Briefly, 3 mL (Position 3) and 10 mL (Positions 9 and 15) Luer lock syringes (Becton Dickinson, Heidelberg, Germany) were used on the syringe actuators. For cartridge separations, a Sep-Pak^® Light Accell^TM Plus QMA Cartridge (Waters, Eschborn, Germany) (preconditioned with 5 mL 1 M K₂CO₃ followed by 10 mL water) and an Oasis^® MCX Plus Extraction Cartridge (Waters) (preconditioned with 5 mL 1 M HCl followed by 5 mL acetonitrile and 10 mL water) were installed on the cassette in Positions 5 and 33, respectively. In Position 35, a Sep-Pak^® Dry Cartridge (unconditioned Na₂SO₄) was installed.

Production of [¹⁸F]AmBF₃-TATE was conducted on a Trasis AllInOne module with 36 manifold actuators and an integrated HPLC system equipped with a Phenomenex Luna C-18 semi-preparative column (100 Å, 10 μm, 10 mm × 250 mm). The cassette setup is shown in Fig. 2 with tubing connections, syringes, and other disposables. The cassettes were pre-assembled in a cleanroom using disposable materials supplied by Trasis. Luer lock syringes (Becton Dickinson) were secured at Positions 3 (3 mL), 9, and 15 (10 mL). A Sep-Pak® Light Accell^TM Plus QMA-Cartridge (Waters) preconditioned with 10 mL saline followed by 20 mL DI water and a Sep-Pak® Light C-18 Cartridge (Waters) preconditioned with 10 mL ethanol followed by 20 mL DI water were installed on Positions 5 and 33, respectively.
Fig. 2

Setup of the synthesis cassette to produce [¹⁸F]AmBF₃-TATE with integrated HPLC purification on Trasis AllInOne module