The total protein was extracted with 1 × SDS loading buffer and boiled for 5 min. The samples were loaded on 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride (PVDF) membrane. Western blot was carried out with anti-GFP antibody (Sigma-Aldrich, G1544) at 1:5,000, anti-mCherry (Sigma-Aldrich, AB356482) at 1:5,000, and anti-tubulin (T6074; Sigma-Aldrich; 1:5,000). The secondary goat anti-rabbit IgG-peroxidase (Sigma–Aldrich, A4914-1ML) was used at 1:5,000 dilution, and goat anti-mouse (A4914-1ML; Sigma–Aldrich) was used at 1:10,000 dilution.
A4914 1ml
Manufactured by Merck Group
A4914-1ML is a laboratory product offered by the Merck Group. It is a 1 mL volume of a substance, but beyond this core function, no further details can be provided in an unbiased and factual manner without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
Anti gfp antibody, by Merck Group (1 mentions)
Anti mcherry, by Merck Group (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Ab32146, by Abcam (1 mentions)
Ab34771, by Abcam (1 mentions)
As132640, by Agrisera (1 mentions)
As08 307a, by Agrisera (1 mentions)
T6074, by Merck Group (1 mentions)
Anti rabbit igg peroxidase, by Merck Group (1 mentions)
2 protocols using a4914 1ml
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of Protein Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein samples were boiled for 5 min. After a 2-min centrifugation at 13,000 rpm, the supernatants were loaded on a 10% SDS-PAGE gel. Rabbit anti-RHD3CT antibody (PHY0765S; PhytoAB; 1:10,000 dilution), anti-AGB1 antibody (PHY0956S; PhytoAB; 1:1000 dilution), anti-LPAT2 antibody (PHY0873S; PhytoAB; 1:1000 dilution), anti-actin antibody (AS13 2640; Agrisera; 1:4000 dilution), anti-GST antibody (AS17 4147; Agrisera; 1:2000 dilution), and anti-ubiquitin antibody (AS08 307A; Agrisera; 1:1000 dilution) were used for the protein gel blot. Mouse anti-tubulin (T6074; Sigma-Aldrich; 1:4000) and goat anti-mouse (A4914-1ML; Sigma-Aldrich; 1:10,000) were used as the loading control of the protein gel blot. For the blotting after immunoprecipitation, a rabbit anti-GFP antibody (ab32146; Abcam) at 1:5000 dilution or a rabbit anti-RFP (ab34771; Abcam) at 1:2000 dilution was used. The secondary anti-rabbit IgG-peroxidase (Sigma-Aldrich) was used at 1:5000 dilution. The intensity of bands was quantified relatively to the reference band by ImageJ.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!