Steviolbioside

Manufactured by ChromaDex

Sourced in United States

Steviolbioside is a natural sweetener compound derived from the Stevia plant. It functions as a sweetening agent, enhancing the flavor of various food and beverage products.

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4 protocols using steviolbioside

Quantification of Steviol Glycosides

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Ethanol and ferrous sulfate were purchased from Carlo Erba SpA (Milan, Italy). Water and acetonitrile were obtained from JT Baker (Phillipsburg, NJ, USA). Pure stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A and steviolbioside (97% pure) were purchased from ChromaDex Inc. (Irvine, CA, USA). Gallic acid monohydrate (3,4,5-trihydroxy benzoic acid), TPTZ (2,4,6-tri(2-pyridyl)-s-triazine), Trizma acetate, Folin–Ciocalteu reagent, sodium carbonate, and ferric chloride were all obtained from Sigma-Aldrich Chemical Co. (Milan, Italy). All chemicals used in the present study, including solvents, were of analytical grade.

Bugliani M., Tavarini S., Grano F., Tondi S., Lacerenza S., Giusti L., Ronci M., Maidecchi A., Marchetti P., Tesi M, & Angelini L.G. (2021). Protective effects of Stevia rebaudiana extracts on beta cells in lipotoxic conditions. Acta Diabetologica, 59(1), 113-126.

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Determination of Steviol Glycosides and Antioxidants

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HPLC-grade acetonitrile and methanol were purchased from VWR (Fontenay-sous-Bois, France) and analytical grade ethanol and ammonium acetate were purchased from Scharlab (Barcelona, Spain). The standards Rebaudioside A, Rebaudioside C, Dulcoside A, Stevioside and Steviolbioside (purity > 98%) were obtained from Chromadex (CA, USA).
De-ionized water from MilliQ (Millipore Corp., Bedford, MA) was used throughout the procedure. Solid-phase extraction (SPE) was carried out on a vacuum manifold system (Lichrolut, Merck, Darmstadt, Germany) using StrataC18-E cartridges (500 mg, 3 mL, 55 μm, 70 Å) from Phenomenex (Torrance, CA) for the determination of steviol glycosides.
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (Sigma-Aldrich, Germany) were used to determine the total antioxidant activity. Sodium nitrite, (+)-catechin, sodium hydroxide (Sigma-Aldrich, Germany) and aluminum chloride hexahydrate (Fluka, Germany) were used to analyse the flavonoids. Sodium carbonate, gallic acid and Folin-Ciocalteu reagent (all purchased from Sigma-Aldrich, Germany) were utilized for phenolic determination.

Periche A., Castelló M.L., Heredia A, & Escriche I. (2015). Influence of Extraction Methods on the Yield of Steviol Glycosides and Antioxidants in Stevia rebaudiana Extracts. Plant foods for human nutrition (Dordrecht, Netherlands), 70(2).

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Quantitative Analysis of Stevia Glycosides

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The HPLC-grade solvents, acetonitrile, formic acid and water were purchased from J. T. Baker (Phillipsburg, NJ, USA). Common Stevia Glycosides Standards Kit (steviolbioside, dulcoside A, rebaudioside B, stevioside, rebaudioside A and rebaudioside C) were purchased from Chromadex (LGC Standards S.r.L., Milan, Italy). All solvents and water were thoroughly degassed prior analyses.

Tavarini S., Passera B., Martini A., Avio L., Sbrana C., Giovannetti M, & Angelini L.G. (2018). Plant growth, steviol glycosides and nutrient uptake as affected by arbuscular mycorrhizal fungi and phosphorous fertilization in Stevia rebaudiana Bert. Industrial Crops & Products., 111.

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Antioxidant Capacity Evaluation Protocol

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Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), fluorescein sodium salt, 2,2′-azobis [2 -methylpropionamide] dihydrochloride (AAPH), quercetin dihydrate, 2′-7′-dichlorodihydrofluorescin diacetate (DCFH-DA), were purchased from Sigma-Aldrich (Milan, Italy). Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose culture media, penicillin, streptomycin, L-glutamine, trypan blue, trypsin, were from culture grade and also purchased from Sigma-Aldrich. Fetal bovine serum (FBS), Dulbecco’s phosphate buffered saline (PBS) without Mg²⁺ and Ca²⁺ and Hank’s balanced salts solution (HBSS) from culture grade were purchased from Euroclone SpA (Milan, Italy). Methanol was purchased from Carlo Erba SpA (Milan, Italy). Water and acetonitrile were obtained from J. T. Baker (Phillipsburg, NJ, USA). A Common Steviol Glycosides Standards Kit (steviolbioside, dulcoside A, stevioside, rebaudioside A, B, and C) was purchased from Chromadex (LGC Standards S.r.L., Milan, Italy). All other chemicals were from analytical high-performance liquid chromatography (HPLC) grade and purchased from common sources. All solvents and water were accurately degassed before being used in the analyses.

Tavarini S., Clemente C., Bender C, & Angelini L.G. (2020). Health-Promoting Compounds in Stevia: The Effect of Mycorrhizal Symbiosis, Phosphorus Supply and Harvest Time. Molecules, 25(22), 5399.

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