Hematoxylin eosin h e stain kit

[2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA), 2-hydroxy-4-(2-hydroxyethoxy)-2-methylpropiophenone (98%), and poly (ethylene glycol) dimethacrylate (PEGDMA, MW = 550 Da) were obtained from Sigma-Aldrich (MO, USA). Poly (ethylene glycol) methyl ether methacrylate (PEG, MW = 475 Da) was obtained from Aladdin (Shanghai, China). A hematoxylin–eosin (H&E) stain kit, BCA assay kit, and Masson’s trichrome (MTS) stain kit were purchased from Beyotime (Beijing, China). Collagen I (ab21286), collagen III (ab7778), laminin (ab11575), fibronectin (ab268020), CD31 (ab28364), VEGF (ab39256), CD68 (ab955), CD163 (ab182422), sQSTM1/p62 (ab56416), TNF-α (ab9755), goat anti-rabbit IgG Alexa Fluor® 647 (ab150083), and goat anti-mouse IgG Alexa Fluor 488® (ab150113) were obtained from Abcam (CA, UK). Matrix metallopeptidase2 (MMP-2) (384993) and laminin (383353) were obtained from Zenbio (Chengdu, China). Microtubule-associated one protein light chain 3 (LC3, L7543) was purchased from Sigma-Aldrich. PI3K (4228), p-PI3K (4257), AKT (4691), p-AKT (4060), mTOR (2983), and p-mTOR (5536) were obtained from Cell Signaling Technologies (Beverly, MA, USA). The RIPA lysis buffer was purchased from GE Healthcare Biosciences (PA, USA). The DAB Chromogen Kit was purchased from ZSGB-BIO (Beijing, China).

Oil-red O powder was purchased from Sigma Aldrich (St. Louis, MO, United States, O0625). All the real-time polymerase chain reaction (PCR) primers were purchased from Sangon (Shanghai, China). Hematoxylin–eosin (H&E) stain kit was purchased from Beyotime Biotechnology Co. (Beijing, China, C0105). RNA extraction kit and real-time PCR kit were purchased from Vazyme Biotech Co., Ltd. (Nanjing, China, Q311). Streptozotocin (STZ) was purchased from Selleck Co. (Shanghai, China, S1312).