The application of cyclic mechanical stress (10% and 0.5 Hz) was carried out with Flexercell FX-5000 Strain Unit (Flexcell International Corporation, Hillsborough, NC, United States) as described previously (Xi et al., 2021 (link)). The PDLSCs were treated with 10% and 0.5 hz cyclic mechanical stress for 0 and 12 h. The PDLSCs in 0 h groups were the non-loaded cells without cyclic mechanical stress. Non-loaded PDLSCs (0 h) were cultured on the Bioflex ® culture plates in the same incubator for the maximum tension time without cyclic mechanical stress.
Flexercell fx 5000 strain unit
Manufactured by Flexcell
Sourced in United States
The Flexercell FX-5000 Strain Unit is a lab equipment designed to apply controlled mechanical strain to cell cultures. It features a computer-controlled vacuum system that creates a cyclical strain on the cells, simulating physiological conditions. The device can be used to study the effects of mechanical forces on various cell types.
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Lab products found in correlation
2 protocols using flexercell fx 5000 strain unit
1
Mechanical Stress Stimulation of PDLSCs
2
Cyclic Mechanical Stress on PDLSCs
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The method was consistent with our previous study.13 (link) PDLSCs at passages 3–6 were cultivated and then seeded in the six-well silicone Bioflex ® culture plates (Flexcell International Corporation, Hillsborough, NC, USA). After PDLSCs reached approximately 80% confluence, 10% and 0.5 Hz cyclic mechanical stress was performed for 3, 12 and 24 h using Flexercell FX-5000 Strain Unit (Flexcell International Corporation).14 (link),15 (link) Non-loaded PDLSCs (0 h) were cultivated and then seeded in the similar culture plates and in the same incubator without cyclic mechanical stress.
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