Ellipse microscope

Manufactured by Nikon

The Ellipse Microscope is a high-quality laboratory instrument designed for detailed observation and analysis. It features a compact and ergonomic design, with a stable and durable construction. The microscope offers advanced optical components, including superior lenses and illumination systems, to provide clear and detailed images. It is suitable for a range of scientific and research applications that require precise magnification and observation capabilities.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Poly l lysine coated coverslips, by BD (1 mentions) Polysine, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Alexa fluor 488 conjugated donkey anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Ellipse inverted microscope Ellipse 80i light microscope Nikon Ellipse 80i microscope Ellipse 800 microscope

2 protocols using ellipse microscope

Quantification of Micronuclei and Chromatin Bridges

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For the quantification of micronuclei, cells were treated with 2 μg ml⁻¹ Cytochalasin B on poly-L-lysine-coated coverslips (BD Bioscience) for 12 h before being fixed and mounted with Vectashield mounting medium with DAPI (Vector laboratories). Micronuclei in binucleated cells were scored using the micronucleus assay21 (link). For the quantification of chromatin bridges and binucleation, cells were harvested following asynchronous growth incubated on Polysine (Thermo Scientific) slides, fixed and mounted with Vectashield mounting medium with DAPI. Slides were scored using a Nikon Ellipse Microscope. Binucleation was defined and scored as in the micronucleus assay.

Nielsen C.F., Huttner D., Bizard A.H., Hirano S., Li T.N., Palmai-Pallag T., Bjerregaard V.A., Liu Y., Nigg E.A., Wang L.H, & Hickson I.D. (2015). PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis. Nature Communications, 6, 8962.

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Immunofluorescent Localization of TRPM8 in BEAS-2B Cells

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BEAS-2B cells cultured in chamber slides were fixed with 4% paraformaldehyde in tris-buffered saline (TBS) for 10 minutes. Cells were washed 3x with TBS containing 0.1% tween-20 (TBS/T), and non-specific binding was blocked using 10% donkey serum and 5% BSA in TBST. The cells were rinsed 3x with TBS and incubated at 4°C for 18 h with a rabbit polyclonal IgG antibody (1:500) specific to human TRPM8 (Abcam, Cambridge, MA). Cells were washed and treated for 1 h at room temperature with an Alexa-Fluor 488 conjugated donkey anti-rabbit IgG secondary antibody (Life Technologies, Carlsbad, CA) diluted 1:400 in the blocking solution. Nuclei were counter-stained with 6-diamidino-2-phenylindole (DAPI) in TBS (1:1000). Negative controls were treated with secondary antibody only. Images were captured at 60X in the green and blue channels with an integrated CMOS camera on a Nikon Ellipse microscope. FITC and DAPI images were merged using ImageJ software.

Nair V., Tran M., Behar R.Z., Zhai S., Cui X., Phandthong R., Wang Y., Pan S., Luo W., Pankow J.F., Volz D.C, & Talbot P. (2020). Menthol in Electronic Cigarettes: A Contributor to Respiratory Disease?. Toxicology and applied pharmacology, 407, 115238.

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