The CD45+MHC-II+CD11b+CD11c+CD103−Gr-1− MP1 cell subset was purified from LPMCs isolated from naïve or C. rodentiun-infected (day 8) mice using a FACSAria cell sorter. FACS purified cells (2 × 105 cells) were lysed in a buffer containing 1% NP-40 supplemented with the complete protease inhibitor cocktail (Roche, Mannheim, Germany) and 2 mM dithiothreitol. Lysates were resolved by SDS-PAGE and transferred to PVDF membranes by electro-blotting. Rat anti mouse-caspase-11 antibody (17D9, Sigma) was used to detect caspase-11 in MPs (1:1000 dilution).
Rat anti mouse caspase 11 antibody 17d9
Manufactured by Merck Group
The Rat anti mouse-caspase-11 antibody (17D9) is a laboratory reagent used for the detection and study of caspase-11 protein in mouse samples. Caspase-11 is an inflammatory caspase involved in the innate immune response. This antibody can be used in various immunological techniques to identify and analyze caspase-11 expression and function.
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Lab products found in correlation
2 protocols using rat anti mouse caspase 11 antibody 17d9
1
Caspase-11 Expression in Intestinal Macrophages
2
Caspase-11 Expression in Mononuclear Phagocytes
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The CD45+MHC-II+CD11b+CD11c+CD103-Gr-1− MP1 cell subset was purified from LPMCs isolated from naive or C. rodentiun-infected (day 8) mice using a FACSAria cell sorter. FACS purified cells (2 × 105 cells) were lysed in a buffer containing 1% NP-40 supplemented with the complete protease inhibitor cocktail (Roche, Mannheim, Germany) and 2 mM dithiothreitol. Lysates were resolved by SDS–polyacrylamide gel electrophoresis and transferred to PVDF (polyvinylidene difluoride) membranes by electro-blotting. Rat anti-mouse-caspase-11 antibody (17D9, Sigma) was used to detect caspase-11 in MPs (1:1000 dilution).
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