Protein mass profiles were obtained using a Microflex LT MALDI-TOF Mass Spectrometer (Bruker Daltonics, Germany), with detection in the linear positive-ion mode at a laser frequency of 50 Hz within a mass range of 2–20 kDa. The setting parameters of the MALDI-TOF MS apparatus were identical to those previously used [43 (link)]. Briefly, the acceleration voltage was 20 kV, and the extraction delay time was 200 ns. Each spectrum corresponds to ions obtained from 240 laser shots performed in six regions of the same spot and automatically acquired using the AutoXecute of the Flex Control v.2.4 software (Bruker Daltonics).
Autoxecute of the flex control v 2
Manufactured by Bruker
Sourced in Germany
The AutoXecute feature of the Flex Control v.2.4 software is a core function that automates the execution of predefined measurement sequences for Bruker's lab equipment. The software enables users to configure and run these automated measurement routines, streamlining the analysis process.
Automatically generated - may contain errors
3 protocols using autoxecute of the flex control v 2
1
MALDI-TOF Mass Profiling of Proteins
2
Mass Profiling of Proteins Using MALDI-TOF
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Protein mass profiles were obtained using a MicroFlex LT MALDI-TOF Mass Spectrometer (Bruker Daltonics, Germany), with detection in the linear positive-ion mode at a laser frequency of 50 Hz within a mass range of 2–20 kDa. The setting parameters of the MALDI-TOF MS apparatus were identical to those previously used [57 (link)]. MS spectra were acquired automatically using the AutoXecute of the Flex Control v.2.4 software (Bruker Daltonics).
3
MALDI-TOF MS Profiling of Mosquito Legs
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Protein mass profiles were acquired using a Microflex LT MALDI-TOF Mass Spectrometer, with detection in the linear positive-ion mode at a laser frequency of 50 Hz within a mass range of 2-20 kDa. The acceleration voltage was 20 kV, and the extraction delay time was 200 ns. Each spectrum corresponded to ions obtained from 240 laser shots performed in six regions of the same spot and automatically acquired using the AutoXecute of the Flex Control v.2.4 software (Bruker Daltonics, Bremen, Germany). The spectrum profiles obtained from mosquito legs and bloody WFPs were visualized with Flex analysis v.3.3 software and were exported to ClinProTools version v.2.2 (Bruker Daltonics, Bremen, Germany) and MALDI-Biotyper v.3.0. (Bruker Daltonics, Bremen, Germany) for data processing (smoothing, baseline subtraction, peak picking) and evaluated using cluster analysis. Spectra of low quality were excluded from the study.
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