Model jsm 5600lv

Samples of parasite vesicles were processed for haematoxylin and eosin (H&E) staining as described^{16 (link),33 (link)} and observed under light microscopy. Samples of cysts were used to SEM examination. The SEM was performed as described^{4 (link),34 (link)}. Briefly, the specimens, isolated vesicles, were fixed in 2.5% glutaraldehyde solution at 4 °C. Subsequently, the samples were stained for 2 h in a 1% osmium tetroxide solution at 4 °C. After washed with PBS buffer, the samples were dehydrated with increasing concentrations of ethanol and then immersed in 2% isoamyl acetate for 20 min. Finally, the specimens were mounted on aluminum stubs and sputter-coated with gold and examined with a scanning electron microscope (Model JSM-5600LV, Japan Electron Optics Laboratory Co. Ltd. Japan).

The mean particle size, polydispersity index (PDI) and zeta potential of PLGA-Fe-E2-a were assessed using Zetasizer Nano ZS ZEN 3,600 (Malvern Instruments Ltd, Malvern, UK). The micrographs of PLGA-Fe-E2-a mounted on aluminum stubs with gold coated were performed at an accelerating voltage of 15–20 kV using scanning electron microscope (Model JSM-5600LV, Japan Electron Optics Laboratory Co. Ltd. Japan).