Quanti luc gold

Manufactured by InvivoGen

QUANTI-Luc Gold is a reagent used to measure and quantify luciferase reporter gene activity. It provides a sensitive and robust detection of luciferase in cell-based assays.

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Lab products found in correlation

Synergy htx multi mode microplate reader, by Agilent Technologies (2 mentions) Pierce protein a g ultralink resin, by Thermo Fisher Scientific (2 mentions) Ht29 lucia ahr cells, by InvivoGen (1 mentions) Microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

QUANTI-LucTM Gold QUANTI-Luc Gold substrate QUANTI-Luc

4 protocols using quanti luc gold

Measuring AhR Signaling Activity

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The activity of the AhR signaling pathway was measured using HT29-Lucia^™ AhR cells, purchased from Invivogen. The cell lines are engineered to study AhR induction by monitoring the activity of Lucia luciferase reporter protein. QUANTI-Luc^™ Gold (InvivoGen) was used to detect the secreted luciferase according to the supplier’s instructions. Briefly, 199 μL cells were seeded at a density of about 50,000 cells/well in a 96 well plate and 1 μL of the desired ligand (or DMSO control) was added. 2-(1H-Indol-3-ylcarbonyl)-4-thiazolecarboxylic acid methyl ester (ITE) was used as a positive control and unstimulated cells were used as a negative control. After a 24 h incubation, 20 μL of cell supernatant was transferred to a black plate and 50 μL of QUANTI-Luc^™ Gold was added immediately prior to reading the luminescence. The promotor activity was repeated in triplicate and values were expressed as fold-change relative to DMSO vehicle treated cells.

Gatsios A., Kim C.S., York A.G., Flavell R.A, & Crawford J.M. (2022). Cellular stress-induced metabolites in Escherichia coli. Journal of natural products, 85(11), 2626-2640.

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sFIS Assay for Interferon-Stimulated Genes

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This sFIS assay [36 (link)] consisted of THP1-Dual™ myeloid reporter cells (InvivoGen, Toulouse, France) seeded in a 96-well plate at a density of 30,000 cells per well in 100 µL media (RPMI-1640 containing 2 mM L-glutamine, 25 mM HEPES, 100 units/mL penicillin, 100 µg/L streptomycin, 100 µg/mL Normocin and 10% heat inactivated fetal calf serum). After 24 h, THP1 cells were treated with 100 µL of patient plasma for 24 h. To determine activation of interferon-stimulated response elements (ISRE) coding for interferon (IFN)-stimulated genes (ISG), luciferase was checked in media by adding 50 µL of Quanti-Luc GOLD (InvivoGen) to 100 µL of (separately recovered) THP1 media. Bioluminescence was examined for 100 ms immediately after Quanti-Luc GOLD addition by microplate reader (Agilent, Santa Clara, CA, USA). IFN/ISG response activity values were normalized using the results of samples taken before RT treatment of each individual patient.

Vaes R.D., Reynders K., Sprooten J., Nevola K.T., Rouschop K.M., Vooijs M., Garg A.D., Lambrecht M., Hendriks L.E., Rucevic M, & De Ruysscher D. (2021). Identification of Potential Prognostic and Predictive Immunological Biomarkers in Patients with Stage I and Stage III Non-Small Cell Lung Cancer (NSCLC): A Prospective Exploratory Study. Cancers, 13(24), 6259.

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Protein A/G Affinity Purification and Detection

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Pierce Protein A/G Ultralink Resin (5 μL; Thermo Fisher Scientific) and 1 μL sample serum in 100 μL Buffer A (50 mM Tris, 150 mM NaCl, 0.1% Triton X-100, pH 7.5) was added to 96-well opaque Multiscreen HTS 96 HV 0.45 μm filter plates (Millipore Sigma). Plates were incubated with shaking at 300 rpm for 1 h at room temperature. Supernatant in wells was removed by centrifugation at 2000 g for 1 min. Luciferase fusion protein (10⁶ RLU) was added to the wells in 100 μL Buffer A. Plates were incubated with shaking at 300 rpm for 1 h at room temperature. Using a vacuum manifold, wells were washed 8 times with 100 μL Buffer A followed by 2 washes with 100 μL PBS. Remaining supernatant in wells was removed by centrifugation at 2000 g for 1 min. Plates were dark adapted for 5 min. An autoinjector equipped Synergy HTX Multi-Mode Microplate Reader (BioTek) was primed with QUANTI-Luc Gold (InvivoGen). Plates were read using the following per well steps: 50 μL QUANTI-Luc Gold injection, 4 second delay with shaking, read luminescence with an integration time of 0.1 seconds and a read height of 1 mm.

Wang E.Y., Dai Y., Rosen C.E., Schmitt M.M., Dong M.X., Ferré E.M., Liu F., Yang Y., González-Hernández J.A., Meffre E., Hinchcliff M., Koumpouras F., Lionakis M.S, & Ring A.M. (2022). High-throughput identification of autoantibodies that target the human exoproteome. Cell Reports Methods, 2(2), 100172.

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Protein A/G Affinity Purification and Luminescence Quantification

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Pierce Protein A/G Ultralink Resin (5 μL; Thermo Fisher Scientific) and 1 μL sample serum in 100 μL Buffer A (50 mM Tris, 150 mM NaCl, 0.1% Triton X-100, pH 7.5) was added to 96-well opaque Multiscreen HTS 96 HV 0.45 mm filter plates (Millipore Sigma). Plates were incubated with shaking at 300 rpm for 1 h at room temperature. Supernatant in wells was removed by centrifugation at 2000 g for 1 min. Luciferase fusion protein (10⁶ RLU) was added to the wells in 100 μL Buffer A. Plates were incubated with shaking at 300 rpm for 1 h at room temperature. Using a vacuum manifold, wells were washed 8 times with 100 μL Buffer A followed by 2 washes with 100 μL PBS. Remaining supernatant in wells was removed by centrifugation at 2000 g for 1 min. Plates were dark adapted for 5 min. An autoinjector equipped Synergy HTX Multi-Mode Microplate Reader (BioTek) was primed with QUANTI-Luc Gold (InvivoGen). Plates were read using the following per well steps: 50 μL QUANTI-Luc Gold injection, 4 second delay with shaking, read luminescence with an integration time of 0.1 seconds and a read height of 1 mm.

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