CD spectra of PARP2FL WT, N127A, N129A, E286A, E286R and G338A were recorded at 22 °C using Chirascan CD spectroscopy (Applied Photophysics Ltd.) equipped with a temperature-regulated sample chamber. The far-UV region spectra (190–280 nm) were measured in a 1 mm path length quartz cuvette. The sample concentration was 0.05–0.07 mg/ml in 10 mM sodium phosphate pH 7.4, 150 mM (NH4)2SO4. The data were analysed with the Pro-Data Software suite (Applied Photophysics Ltd.).
Chirascan cd spectroscopy
Manufactured by Applied Photophysics
Sourced in United Kingdom
The Chirascan CD spectroscopy system is a laboratory instrument designed to measure the circular dichroism (CD) of samples. CD spectroscopy is a technique used to analyze the chirality and secondary structure of biomolecules, such as proteins and nucleic acids. The Chirascan system provides accurate and reliable measurements of CD spectra across a wide range of wavelengths, enabling researchers to study the structural properties of their samples.
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Lab products found in correlation
2 protocols using chirascan cd spectroscopy
1
CD Spectroscopy of PARP2 Mutants
2
Probing BSA structure via CD spectroscopy
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The CD spectra of BSA solution in the absence or presence of ECG with different ultrasonic treatment were recorded in the range of 200-260 nm at 298 K by the Chirascan CD spectroscopy instrument (Applied Photophysics Ltd., UK) with a 1 mm cell. The concentration of BSA was kept at 1.0 Â 10 À6 molÁL À1 and the molar ratios of ECG to BSA were varied from 0:1 to 2:1. The corresponding contribution of the model wine solution to the spectra was recorded and digitally subtracted to the spectra at the same ultrasonic conditions. The compositions of the secondary structure on free proteins and ECG-binded complex were estimated according to the CD spectra [33] .
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