Sybr green qpcr kit

Manufactured by Tiangen Biotech

Sourced in China

The SYBR Green qPCR kit is a reagent used for quantitative real-time polymerase chain reaction (qPCR) analysis. It contains SYBR Green, a fluorescent dye that binds to double-stranded DNA, enabling the detection and quantification of target DNA sequences during the amplification process.

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Lab products found in correlation

Reverse transcription kit, by Takara Bio (1 mentions) Rnasimple kit, by Tiangen Biotech (1 mentions) Fast king rt reagent kit with gdna eraser, by Tiangen Biotech (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Mx3005p qpcr system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Talent qPCR PreMix (SYBR Green) Kit MiRcute Plus miRNA qPCR Detection Kit (SYBR Green) MiRcute Plus miRNA qPCR Kit (SYBR Green) MiRcute plus microRNA SYBR Green qPCR Kit LnRcute lncRNA qPCR Kit (SYBR Green; MiRcute miRNA qPCR Detection Kit (SYBR Green; FastFire qPCR PreMix (SYBR Green) Kit MiRNA qPCR Detection Kit (SYBR Green) QPCR Detection Kit (SYBR Green; SYBR qPCR Kit

2 protocols using sybr green qpcr kit

Quantification of Circular RNA and miRNA Expression

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Total RNA of LAUD tumor tissues and paired noncancer tissues or LAUD cell lines and HBE cells was extracted by using RNAsimple kit (Tiangen, Beijing, China), followed by reverse-transcribed to cDNA via a Reverse Transcription kit (Takara Biotechnology Co., Ltd.). Quantitative RT-PCR was conducted with the SYBR Green qPCR kit (Tiangen, Beijing, China) and 2^−ΔΔCq method was employed to calculate the relative RNA expression. GAPDH and U6 were used as an internal control. The sequences of the primers used in this study were as follows:
circ-ZNF609:
Forward: 5ʹ-GCCACACTACCAGACAACATC-3ʹ
Reverse: 5ʹ-TAATACGACTCACTATAGGGAACCGGCTACACTGCGG-3ʹ
hsa-miR-1224-3p:
Forward: 5ʹ- CCCCACCTCCTCTCTCCTCAG-3ʹ
ETV1:
Forward: 5ʹ-TGGCAGTTTTTGGTAGCTCTTC-3ʹ
Reverse: 5ʹ-CGGAGTGAACGGCTAAGTTTATC-3ʹ
GAPDH:
Forward: 5ʹ-GGAGCGAGATCCCTCCAAAAT-3ʹ
Reverse: 5ʹ-GGCTGTTGTCATACTTCTCATGG-3ʹ
U6:
Forward: 5ʹ- GCTTCGGCAG CACATATACTAAAAT-3ʹ
Reverse: 5ʹ- CGCTTCACGAATT TGCGTGTCAT-3ʹ

Zuo Y., Shen W., Wang C., Niu N, & Pu J. (2020). Circular RNA Circ-ZNF609 Promotes Lung Adenocarcinoma Proliferation by Modulating miR-1224-3p/ETV1 Signaling. Cancer Management and Research, 12, 2471-2479.

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Aorta Tissue RNA Extraction and qPCR

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Total RNA from the entire aorta tissue was extracted by Trizol reagent according to the manufacture’s protocol (Invitrogen^TM, Carlsbad, CA, USA). The purified RNA was reverse-transcribed to cDNA using the FastKing RT reagent kit with gDNA Eraser (Tiangen Biotech, Beijing, China). PCR reactions were performed by the SYBR Green^® qPCR kit (Tiangen Biotech, Beijing, China) on an Mx3005P qPCR system (Agilent Technologies, Palo Alto, CA, USA), and normalized to Gapdh. The sequences of the primers were shown in Table S1.

Liu C., Feng R., Zou J., Xia F, & Wan J.B. (2019). 20(S)-Protopanaxadiol Saponins Mainly Contribute to the Anti-Atherogenic Effects of Panax notoginseng in ApoE Deficient Mice. Molecules, 24(20), 3723.

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