Ca free keratinocyte sfm medium k sfm

Active reagents and the concentrations used were selected from previous TPMD literature^{7 (link),35 (link)–37 (link)} and from the TRP channel literature^{38 (link)}. AMG 9810, AMTB, ryanodine, BAPTA-AM, 18-α glycyrrhetinic acid (18α-GA), apyrase and DAPI were purchased from Sigma-Aldrich, St. Louis, MO; thapsigargin, BCTC, and Cal-520-AM dye were purchased from Abcam, Cambridge, United Kingdom, Focus Biomolecules, Plymouth Meeting, PA, and AAT Bioquest, Sunnyvale, CA, respectively. Polyclonal connexin 43 antibody was purchased from Cell Signaling, Danvers, MA; Alexa Fluor 488 was purchased from Fisher, Pittsburgh, PA. Ca⁺⁺-free Keratinocyte-SFM medium (K-SFM) was purchased from ThermoFisher Scientific (Cat. No.: 10725–018), Waltham, MA.

Reagents and the concentrations used were selected from previous TPMD literature [3 (link), 11 (link)–14 (link)] and from the TRP channel literature [15 (link)]. Dulbecco’s modified Eagle’s medium (DMEM) and Ca⁺⁺-free Keratinocyte-SFM medium (K-SFM), and alexa fluor 488 dextran (FDx) were purchased from ThermoFisher Scientific, Waltham, MA. Cal-520-AM dye was purchased from AAT Bioquest, Sunnyvale, CA. Cyclopiazonic acid (CPA), 18-α glycyrrhetinic acid (18α-GA), apyrase, AMG 9810, x-methyltryptophan (AMTB) and ryanodine were purchased from Sigma-Aldrich, St. Louis, MO. Thapsigargin was purchased from Abcam, Cambridge, United Kingdom, and N-(4-tertiarybutylphenyl)-4-(3-cholorphyridin-2-yl) tetrahydropyrazine-1(2H)-carbox-amide (BCTC) was purchased from Focus Biomolecules, Plymouth Meeting, PA.