Fusion 360 cad

PLA models were designed using the Fusion 360 CAD program from Autodesk (California, USA), as described in our previous works [22 (link),28 ]. Briefly, well plates with a customized number of wells were designed with a 150 μL volume capacity for each well. Accordingly, microreactors with a serpentine-like internal structure were designed with a 150 μL working volume. Examples of a nine-well plate and a microreactor model are presented in Figure 1. The final designs were forwarded to the slicing software Cura (Ultimaker, Utrecht, The Netherlands), where appropriate slicing settings were applied, depending on the filament type and the 3D printer used. The printing parameters set by the slicing software are summarized in Table 1. The final printed models were leak-free and ready to use for further experimentation. This was confirmed by filling the printed models with buffer solution and leaving them for 24 h at room temperature. At the end of this period, the total volume collected was the same as the total volume loaded, so no leakage was observed. Additionally, when different flow rates were applied in the microreactor, the total volumes measured at the input and output of the microreactor were identical.

All 3D models for the 3D printer upgrade were prepared in the Fusion360 CAD program (Autodesk) and printed using a UNI 3D printer. The sample models we wanted to print were prepared in the Fusion360 CAD program as solid blocks with dimensions of 10 × 10 × 0.01 mm and saved as an .stl file. Obtained models were sliced in an IdeaMaker slicer program (Raise3D) with the following parameters: extrusion width = 0.3 mm; bottom and top solid fill layers = 0; infill density = 50%; printing speed = 20 mm/s; travel speed = 100 mm/s, “Rectilinear” infill pattern type. The obtained g-code was used without any modifications.