Klebsiella pneumoniae k pneumoniae

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Klebsiella pneumoniae (K. pneumoniae) is a Gram-negative, rod-shaped bacterium belonging to the Klebsiella genus. It is a common inhabitant of the human gastrointestinal tract and can also be found in the environment. K. pneumoniae is known for its ability to cause a variety of infections, including pneumonia, urinary tract infections, and bloodstream infections, especially in immunocompromised individuals.

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Klebsiella pneumoniae (153 citations) K. pneumoniae (52 citations)

3 protocols using klebsiella pneumoniae k pneumoniae

Antimicrobial Lipid Nanoparticle Formulation

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Hydrogenated l-α-phosphatidylcholine (Hydro Egg PC), cholesterol, (N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-phytosphingosine (C6-NBD) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-RhB) were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). Mueller-Hinton broth (MHB) and Mueller-Hinton agar (MHA) were purchased from Oxoid (Basingstoke, England). Melittin was obtained from Aladdin (Shanghai, China). Methicillin-resistant S. aureus (MRSA, ATCC 33591), vancomycin-resistant E. faecalis (VRE, ATCC 700802), Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853), and Klebsiella pneumoniae (K. pneumoniae, ATCC 13383) were obtained from ATCC (USA) and reconstituted according to the suggested protocols. Triton X-100 was purchased from Sigma-Aldrich (Shanghai, China). Dulbecco's Modified Eagle Medium (DMEM), Fetal Bovine Serum (FBS), and 1% penicillin/streptomycin were purchased from Gibco Life Technologies (Grand Island, NY, USA). All other chemicals and solvents were of analytical grade and used without further purification.

Shi Y., Feng X., Lin L., Wang J., Chi J., Wu B., Zhou G., Yu F., Xu Q., Liu D., Quan G., Lu C., Pan X., Cai J, & Wu C. (2021). Virus-inspired surface-nanoengineered antimicrobial liposome: A potential system to simultaneously achieve high activity and selectivity. Bioactive Materials, 6(10), 3207-3217.

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Antimicrobial Evaluation of Clinical Isolates

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In vitro assays were performed against 32 clinical isolates (16 Gram-positive and 16 Gram-negative strains), from the bacterial library of the Department of Biomedical and Biotechnological Sciences (University of Catania, Catania, Italy). Staphylococcus aureus (S. aureus; ATCC 29213), Staphylococcus epidermidis (S. epidermidis; ATCC 14990), Enterococcus faecalis (E. faecalis; ATCC 29212), Enterococcus faecium (E. faecium; ATCC 700221), Escherichia coli (E. coli; ATCC 35218), Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (K. pneumoniae; ATCC 700630) and Proteus mirabilis (P. mirabilis; ATCC 7002) were purchased from ATCC and used as reference strains.

Genovese C., Cambria M.T., D'angeli F., Addamo A.P., Malfa G.A., Siracusa L., Pulvirenti L., Anfuso C.D., Lupo G, & Salmeri M. (2020). The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth. International Journal of Oncology, 57(5), 1129-1144.

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Synthesis and Characterization of CoAEMAPF6

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2-Cobaltoceniumamidoethyl methacrylate hexafluorophosphate (CoAEMAPF₆) was synthesized according to our earlier reports[52 (link), 56 (link)]. 2-Aminoethyl methacrylate hydrochloride (90%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC-HCl, 98%), 4-(dimethylamino) pyridine and tetrabutylammonium chloride (TBACl) were purchased from Aldrich and used as received. Water was from Thermo Scientific Nanopure with ion conductivity at 18.2 MΩ. The following bacterial strains were purchased from ATCC: Staphylococcus aureus (S. aureus, ATCC-33591), Bacillus cereus (B. cereus, ATCC 11778), Escherichia coli (E. coli, ATCC-11775), Klebsiella pneumoniae (K. pneumoniae, ATCC-35596), Proteus vulgaris (P. vulgaris ATCC 33420). Nitrocefin was purchased from TOKU-E and used as received. Sodium salt of penicillin-G was purchased from VWR and used as received. 4-Cyanopentanoic acid dithiobenzoate (CPDB) was obtained from Strem Chemical Inc. Azobisisobutyronitrile (AIBN) was recrystallized from methanol before use. All other chemicals and reagents were from commercial sources and used as received.

Pageni P., Yang P., Bam M., Zhu T., Chen Y.P., Decho A.W., Nagarkatti M, & Tang C. (2018). Recyclable Magnetic Nanoparticles Grafted with Antimicrobial Metallopolymer-Antibiotic Bioconjugates. Biomaterials, 178, 363-372.

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