Cells were stained by fluoro-conjugated antibodies for 30 min at 4 °C. After staining, cells were washed with cold PBS for several times, and were resuspended with PBS containing 2% FBS. Cells were analyzed on a FACSCanto II, a FACSAria III or a FACSAria Fusion using the FACSDiva software (BD Biosciences). Analyses were performed using FlowJo (BD). Dead cells were eliminated by DAPI (BioLegend, catalog #422801) or 7-AAD (Miltenyi Biotec, 130-111-568). The following fluoro-conjugated antibodies were used in this study at indicated dilutions: anti-human Lineage Cocktail BV510 (BioLegend, 348807, clone OKT3; M5E2; 3G8; HIB19; 2H7; HCD56, 1:100), anti-human CD34-APCCy7 (BioLegend, 343513, clone 581, 1:500), anti-human CD38-PECy7 (BioLegend, 303515, clone HIT2, 1:500), anti-human CD90-biotin (BioLegend, 328106, clone 5E10, 1:500), anti-human CD45RA-FITC (BioLegend, 304105, clone HI100, 1:100), anti-human CD10-PE (BioLegend, 312204, clone HI10a, 1:100 for sorting and 1:500 for analysis), Streptavidin-BV605 (BioLegend, 405229, 1:500), anti-human CD34-PECy7 (BioLegend, 343616, clone 561, 1:500), anti-human CD19-APC (BioLegend, 302212, clone HIB19, 1:500), anti-human CD19-APC (Miltenyi Biotec, 130-091-248, 1:33), anti-human CD45 VioBlue (Miltenyi Biotec, 130-113-122, clone 5B1, 1:33).
Anti human cd45 vioblue
Manufactured by Miltenyi Biotec
The Anti-human CD45 VioBlue is a fluorochrome-conjugated monoclonal antibody that binds to the CD45 protein expressed on the surface of human leukocytes. It is designed for use in flow cytometry applications to identify and enumerate various leukocyte subpopulations.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin bv605, by BioLegend (1 mentions)
Anti human cd34 pe cy7, by BioLegend (1 mentions)
Anti human cd38 pecy7, by BioLegend (1 mentions)
Anti human cd45ra fitc, by BioLegend (1 mentions)
Apc anti human cd19, by BioLegend (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions)
Facscanto 2, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
7 aad, by Miltenyi Biotec (1 mentions)
Facschorus software, by BD (1 mentions)
Facsmelody, by BD (1 mentions)
2 protocols using anti human cd45 vioblue
1
Multiparametric Flow Cytometry for Hematopoietic Stem Cell Isolation
2
Multicolor Flow Cytometry Profiling
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Flow cytometry measurements were performed using BD FACS Chorus software on a BD FACS Melody and analyzed using FlowJo 10.6.1 (FlowJo Engine, Becton Dickinson & Company). Cells were detached, washed three times with DPBS and stained with conjugated antibodies for 30 min at 4 °C. Subsequently, cells were washed three times with DPBS and resuspended in PBS + 1% FCS for immediate analysis. The following conjugated antibodies were used: anti-human SSEA-4 PE-Vio770 (Miltenyi Biotech, catalog no. 130-105-081), anti-human CD11b FITC (Thermo Fisher Scientific, catalog no. 11-0118-42) and anti-human CD45 VioBlue (Miltenyi Biotech, catalog no. 130-110-775). Doublets were excluded in FSC and SSC. Unstained cells served as negative population.
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