Mpeg amine

Manufactured by Creative PEGWorks

Sourced in United States

MPEG-amine is a polyethylene glycol (PEG) derivative with an amine functional group. It is a water-soluble compound commonly used as a linker or spacer in various bioconjugation applications.

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Lab products found in correlation

Deuterated solvent, by Cambridge Isotopes (1 mentions) Doxorubicin, by LC Laboratories (1 mentions) Fluosphere, by Thermo Fisher Scientific (1 mentions)

3 protocols using mpeg amine

Hydrophobic Bioink for Drug Delivery

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The hydrophobic bioink was formulated as previously described [24 (link)], with minor modifications. Briefly, the bioink was composed of 30% poly(ethylene glycol) diacrylate (PEGDA, 250 Da), 50% PEG200, and 20% ethanol. Eosin Y (1.0 mM) and mPEG-amine (0.05 M) (350 Da, Creative PEG Works, Chapel Hill, NC, USA) were added as photoinitiator and co-initiator, respectively. Spironolactone was added at a concentration of 20 mg/mL. All chemicals were purchased from Sigma–Aldrich unless otherwise stated.

Acosta-Vélez G.F., Linsley C.S., Zhu T.Z., Wu W, & Wu B.M. (2018). Photocurable Bioinks for the 3D Pharming of Combination Therapies. Polymers, 10(12), 1372.

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Synthesis of Functionalized Organic Dyes

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All starting materials and solvents were purchased from Sigma-Aldrich, TCI Chemicals, or Fisher Scientific and, unless otherwise specified, were used without further purification. Deuterated solvents were purchased from Cambridge Isotope Laboratories. mPEG-Amine and mPEG-OC18 (MW 2000 Da) were purchased from Creative PEGWorks. Doxorubicin, hydrochloride salt was purchased from LC Laboratories. 9,10-dimethoxyanthracene,⁴⁵ 9-methoxyanthracene,⁴⁶ cis-1,2-diphenyldithioethene⁴⁷ were synthesized according to literature procedures.

Brega V., Scaletti F., Zhang X., Wang L.S., Li P., Xu Q., Rotello V.M, & Thomas SW I.I.I. (2019). Polymer Amphiphiles for Photoregulated Anticancer Drug Delivery. ACS applied materials & interfaces, 11(3), 2814-2820.

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Functionalized Nanoparticle Conjugation

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Table 1 lists the vendors and catalog numbers for materials used in these studies. Carboxylatemodified FluoSpheres™ and QDot™ nanoparticles were purchased from ThermoFisher. mPEGamine (molecular weight (MW): 2,000 g/mol) and azide-PEG-amine (MW: 2048 g/mol) were from Creative PEGWorks. DNA conjugation annealing handles and probes were purchased from Integrated DNA Technologies (IDT) or Eurofins Scientific; sequences are listed in Table 3.

Stawicki C., Rinker T.E., Burns M., Tonapi S.S., Galimidi R.P., Anumala D., Robinson J.K., Klein J.S., & Mallick P. (2021). Modular Fluorescent Nanoparticle DNA Probes for Detection of Peptides and Proteins.

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