Log In Sign up
The largest database of trusted experimental protocols

Agilent 244k microarray

Manufactured by Agilent Technologies
Visit Supplier

The Agilent 244K microarray is a high-density microarray platform designed for genome-wide analysis. It features 244,000 probes that can be used to interrogate gene expression, copy number variations, and other genomic features across the entire genome.

Automatically generated - may contain errors

Lab products found in correlation

Glutathione sepharose 4b beads, by GE Healthcare (1 mentions) Sybr green based fluorescence, by Bio-Rad (1 mentions)

Spelling variants of this product

Agilent microarrays (24 citations) Agilent 244K (6 citations) 244k microarray (2 citations) Agilent Human Genome CGH Microarray Kit 244K (2 citations) Agilent Human Genome Microarray Kit 244K (2 citations)

2 protocols using agilent 244k microarray

1

Identifying ARGLU1 RNA Interactors

Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedure was performed as previously described (23 ). Briefly, full-length or truncated GST-tagged ARGLU1 proteins were purified from bacteria (23 ). Individual proteins (20 pmol) and RNA pool (1.5 nmol) were incubated in 1 mL of binding buffer (20 mM HEPES pH 7.8, 80 mM KCl, 20 mM NaCl, 10% glycerol, 2 mM DTT, 0.1 mg/ml BSA) containing 20 μl of glutathione sepharose 4B beads (GE Healthcare, pre-washed three times in binding buffer) for 30 min at 4°C. Beads were then washed and the recovered RNA was directly labeled with either Cy3 or Cy5 and subsequently hybridized to the Agilent 244K microarray used to generate the RNA pool. One-sided Z-scores were calculated for the motifs as previously described (23 ).
Magomedova L., Tiefenbach J., Zilberman E., Le Billan F., Voisin V., Saikali M., Boivin V., Robitaille M., Gueroussov S., Irimia M., Ray D., Patel R., Xu C., Jeyasuria P., Bader G.D., Hughes T.R., Morris Q.D., Scott M.S., Krause H., Angers S., Blencowe B.J, & Cummins C.L. (2019). ARGLU1 is a transcriptional coactivator and splicing regulator important for stress hormone signaling and development. Nucleic Acids Research, 47(6), 2856-2870.
+ Open protocol
+ Expand
2

Quantification of AXIN2 Expression in Glioblastoma

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The abundance of mRNA transcripts was assessed by conversion into complementary DNA and subsequent relative quantification using SYBR green-based fluorescence (Bio-Rad, Hercules, CA). Real-time PCR normalized to the housekeeping gene β-actin was performed with the △△Ct-method. Primer sequences can be found in Supporting Information File S2. Western blotting was performed as described before (60 (link)), and antibodies were used as per the manufacturer’s instructions (Supporting Information File S2).
For TCGA analyses, AXIN2 transcription levels acquired using the Agilent 244k microarray in 401 GBM specimens collected in The Cancer Genome Atlas ([TCGA], http://cancergenome.nih.gov) were retrieved through cBioPortal (http://www.cbioportal.org [61 (link)]) from the provisional Glioblastoma dataset in May 2015. AXIN2 expression values were correlated with available overall survival data provided by TCGA, with a cutoff of 1.75 standard deviations above the median (z-score) considered as AXIN2 high-expressing tumors.
Kahlert U.D., Suwala A.K., Koch K., Natsumeda M., Orr B.A., Hayashi M., Maciaczyk J, & Eberhart C.G. (2015). Pharmacological WNT Inhibition Reduces Proliferation, Survival and Clonogenicity of Glioblastoma Cells. Journal of neuropathology and experimental neurology, 74(9), 889-900.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!