Leukocyte subsets were identified using an antibody panel containing CD45-PO (Life Technologies) for lymphocytes, CD3-AF 700 (BioLegend) for T cells, CD14-PeCy7 (BioLegend) for monocytes, CD19-BV711 (BioLegend) for B cells, and CD16/CD56-APC (eBioscience, BD Biosciences) for NK cells. T cell subsets were distinguished by CD3-AF 700 (BioLegend), CD4-BV711 (BioLegend), CD8-PeCy7 (BD), CD27-APC-eF780 (eBioscience, San Diego, CA, USA), and CD45RO-PB (BioLegend). Each sample was also stained with isotype-matched control monoclonal antibodies for spectral compensation and to correct for background fluorescence. Because of the extensive antibody panel, some antibodies were measured in the same fluorescence channel, e.g., CD4 and CD19, CD8 and CD14, and CD16 and CD56. A representative example of the gating strategy to identify the different lymphocyte subsets is provided in Figure S1 in Supplementary Material.
Cd19 bv711
Manufactured by BioLegend
CD19-BV711 is a fluorochrome-conjugated antibody that binds to the CD19 cell surface antigen. CD19 is a B cell-specific surface receptor that plays a role in B cell activation and differentiation. The BV711 fluorochrome can be used for multicolor flow cytometry applications.
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Lab products found in correlation
Cd27 apc ef780, by Thermo Fisher Scientific (1 mentions)
Cd14 pe cy7, by BioLegend (1 mentions)
Cd45 po, by Thermo Fisher Scientific (1 mentions)
Cd3 af700, by BioLegend (1 mentions)
Cd4 bv711, by BioLegend (1 mentions)
Cd8 pe cy7, by BD (1 mentions)
Cd16 32, by BD (1 mentions)
Ly6g pe, by BioLegend (1 mentions)
Cd3 bv711, by BioLegend (1 mentions)
Cd62l bv650, by BioLegend (1 mentions)
Cd11b af700, by BioLegend (1 mentions)
Zombie aqua, by BioLegend (1 mentions)
Cd45 apc cy7, by BioLegend (1 mentions)
Facsaria 2, by BD (1 mentions)
2 protocols using cd19 bv711
1
Comprehensive Immune Cell Profiling Protocol
2
Murine Lung Immune Cell Profiling
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On day 7, the lungs were isolated from mice, grounded, and subsequently passed through a 70μm cell strainer. Ammoniumchloride-potassium lysing buffer was used for erythrocyte lysis. Multiparameter assessments were performed using BD FACSAria II (BD Biosciences) and data was analyzed with Flowjo software (version 10.6). After staining Zombie Aqua™ (Biolegend) to exclude dead cells, single-cell suspensions were incubated with the fluorochrome-conjugated antibodies in PBS containing 2% fetal bovine serum and then washed twice before detection. Antibodies specific to mouse used included CD16/32 (BD, 553,141), CD45-APC-Cy7 (Biolegend, 103,116), CD11b-AF700 (Biolegend, 101,222), Ly6G-PE (Biolegend, 127,608), CD62L-BV650 (Biolegend, 104,453), CD3-BV711 (Biolegend, 100,241), CD19-BV711 (Biolegend, 115,555), NK1.1-Pacific Blue (Biolegend, 109,816).
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