Immunohistochemical staining performed on 4-μm, formalin-fixed, paraffin-embedded sections using caspase 3 antibodies at 1:50 dilution (DAKO, Carpinteria, CA). Antigen retrieval was performed in all cases by steam heating the slides in a 1-mmol/L solution of EDTA (pH 8.0) for 30 minutes. After blocking of endogenous biotin, staining was performed using an automated immunostainer (DAKO) followed by detection by using a streptavidin-biotin detection system (DAKO). Positive and negative control sections used for each assay.
Streptavidin biotin detection system
Manufactured by Agilent Technologies
Sourced in Denmark
The Streptavidin-biotin detection system is a laboratory equipment product that facilitates the detection and analysis of biomolecular interactions. It utilizes the high-affinity binding between streptavidin and biotin to enable sensitive and specific detection of target analytes.
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Lab products found in correlation
2 protocols using streptavidin biotin detection system
1
Caspase 3 Immunostaining in FFPE Tissues
2
Immunohistochemical Localization of PCNA
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Paraffin sections were deparaffinised in xylene and hydrated. Sections were incubated with primary antibodies after stopping the reaction of peroxidase by adding 10% hydrogen peroxide. Proliferating cell nuclear antigen (PCNA) antibody is a mouse monoclonal antibody at 1:400 dilution (Sigma-Aldrich, St. Louis, Missouri, USA). Antigen retrieval was applied for 30 min by steam heating the slides in a 1-mmol/L solution of EDTA (pH 8.0). After stopping of endogenous biotin reaction, staining was carried out by an automated immunostainer then by applying a streptavidin-biotin detection system (Dako, Glostrup, Denmark). Sections were counterstained with haematoxylin. Primary antibody (as a negative control) was changed by PBS [29] .
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