Pa200 guard column

The chemical composition analysis of pretreated solid fractions (lignin, glucose, xylose, galactose, mannose and arabinose) was performed according to the analytical procedure provided by the National Renewable Energy Laboratory (NREL/TP-510-42618 [67 ]), and the carbohydrates in supernatant were then quantified by high performance liquid chromatography (HPLC) (Agilent, Palo Alto, CA, USA). The HPLC system fitted with a Bio-Rad Aminex HPX-87H column was operated at 55 °C with 5 mM H₂SO₄ as the mobile phase at the flow rate of 0.6 mL/min as previously described by Chen et al. [68 (link)].
HPAEC-PAD analysis was performed on a Dionex ICS-5000 system (Dionex, Sunnyvale, CA, USA) equipped with pulsed amperometric detection (PAD) and a CarboPac PA200 analytical column (3 × 250 mm) with a CarboPac PA200 guard column (3 × 50 mm) according to Shi et al. described method [13 (link)]. MALDI-TOF MS analysis was performed on a 5800 MALDI TOF/TOF analyzer (AB SCIEX, Foster City, CA, USA) equipped with neodymium: yttrium-aluminum-garnet laser (laser wavelength was 349 nm) according to Shi et al. described method [13 (link)].

HPAEC analysis was performed on a ICS-3000 HPLC (Dionex Corporation) using a CarboPac PA200 analytical column (3 × 150 mm) and a CarboPac PA200 guard column (3 × 30 mm) at 30 °C. Following injection of 25 μL of diluted samples, elution was performed at 0.4 mL∕ min using 0.1 M NaOH in the mobile phase with sodium acetate gradients. For aldonic acid separation, the acetate gradients were 0 mM for 1 min, increasing to 80 mM in 8 min, increasing to 300 mM in 2 min, keeping at 300 mM for 1 min, increasing to 600 mM for 2 min, keeping at 600 mM for 5 min, and re-equilibrating at 0 mM for 4 min. Elution was monitored using pulsed amperometric detection (PAD) and peaks were analyzed and quantified using the Chromeleon software package.