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L ascorbic acid

Manufactured by Sisco Research Laboratories
Sourced in India

L-ascorbic acid is a white, crystalline organic compound that is widely used in laboratory research. It is the most biologically active form of vitamin C and serves as an essential nutrient, antioxidant, and cofactor in various enzymatic reactions.

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5 protocols using l ascorbic acid

1

Antioxidant Capacity Determination Protocol

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Folin-Ciocalteu reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K3Fe (CN)6), trichloroacetic acid (TCA), ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.
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2

Antioxidant Capacity Evaluation Protocol

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Folin-Ciocalteu (FC) reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, Gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K3Fe (CN)6), trichloroacetic acid (TCA) and ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.
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3

Antioxidant Activity Assay Protocol

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Folin-Ciocalteu reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, Gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K 3 Fe (CN) 6 ), trichloroacetic acid (TCA), ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.
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4

Synthesis of Graphite-Ilmenite Composite

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Ilmenite sand with a particle size of 100–200 µm was supplied by Lanka Mineral Sand (Pvt) Ltd. Graphite was obtained from Bogala, Sri Lanka. Hydrochloric acid (37%, HCl) and 2-propanol (99.95%, C3H7OH) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Ammonium hydroxide (29%, NH4OH) was purchased from Merk & Co., Inc., Rahway, NJ, USA. Ferrous sulfate heptahydrate (99.5%, FeSO4.7H2O) and L-ascorbic acid (C6H8O6) were sourced from Sisco Research Laboratories in India. Methylene Blue was bought from Daejung Chemical & Metal Co., Ltd. (Shiheung, South Korea). All reagents used were analytical grade and solutions were prepared using ultra-pure water.
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5

Assay of Antioxidant and Enzymatic Activities

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Pyrogallol, Catalase, reduced glutathione (GSH), 2,2- diphenyl-1-picrylhydrazyl (DPPH), 1-chloro-2,4-dinitrobenzene (CDNB), Acetylthiocholine chloride, and 5,5-dithio-bis-2-nitrobenzoic acid (DTNB) were procured from Sigma Chemical Co. (St. Louis, MO, USA); hydrogen peroxide, sodium hydroxide, sodium di-hydrogen phosphate, L-ascorbic acid, and sodium carbonate were obtained from Sisco Research Laboratory, Mumbai, India. Sodium Chloride, Magnesium Chloride, and Tris-base were purchased from Himedia Laboratories, Mumbai.
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